Shiseido Global Innovation Center, Yokohama, Japan.
Graduate Department of Electronics, Tohoku Institute of Technology, Sendai, Japan.
Sci Rep. 2020 Jun 15;10(1):9626. doi: 10.1038/s41598-020-66723-1.
Oxidative stress is associated with skin ageing and disease in humans. However, it is difficult to evaluate the effects of oxidative stress on the skin in vivo using conventional invasive methods. In this study, we performed two-dimensional imaging of ultra-weak photon emission (UPE) generated by excited species in oxidative reaction to determine regional variations in oxidative stress in human facial skin and analysed the relationship between UPE intensity and biophysical properties in vivo. UPE imaging of the facial skin of volunteers revealed regional variations in oxidative stress. The nose, its surrounding regions, and the area between eyebrows showed higher UPE intensity than other facial regions, indicating high oxidative stress in these regions. In contrast, only the region surrounding the eyes showed age-related alterations in UPE intensity; moreover, wrinkle score in these regions was correlated with UPE intensity. These results suggest that oxidative stress in the skin induces wrinkle formation. UPE intensity was correlated with porphyrin score in the skin; however, no correlation was observed between UPE intensity and skin colour parameters. This study provides insights into the treatment of facial skin areas vulnerable to ageing and helps improve our understanding of topical skin diseases related to oxidative stress.
氧化应激与人类皮肤衰老和疾病有关。然而，使用传统的侵入性方法很难评估氧化应激对皮肤的体内影响。在这项研究中，我们对氧化反应中激发态产生的超弱光发射（UPE）进行二维成像，以确定人体面部皮肤中氧化应激的区域变化，并分析 UPE 强度与体内生物物理特性之间的关系。志愿者面部皮肤的 UPE 成像显示出氧化应激的区域变化。鼻子、其周围区域和眉毛之间的区域显示出比其他面部区域更高的 UPE 强度，表明这些区域存在高氧化应激。相比之下，只有眼睛周围的区域显示出与年龄相关的 UPE 强度变化；此外，这些区域的皱纹评分与 UPE 强度相关。这些结果表明，皮肤中的氧化应激会导致皱纹形成。UPE 强度与皮肤中的卟啉评分相关；然而，UPE 强度与皮肤颜色参数之间没有相关性。本研究深入了解了对面部皮肤易老化区域的治疗方法，并有助于提高我们对与氧化应激相关的局部皮肤疾病的认识。
