Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Biochem Genet. 2022 Dec;60(6):2587-2610. doi: 10.1007/s10528-022-10237-0. Epub 2022 Jun 7.
Ducrosia anethifolia Boiss is an aromatic vegetable and medicinal plant of Apiaceae family. In this study, morphological and essential oil studies as well as ISSR analyses were employed to investigate genetic diversity in 120 Moshgak accessions of 24 Iranian populations. High variations were observed in morpho-physiological traits (morphological and essential oil contents) of the populations in 2 consecutive agronomic years. In both studied years, the highest leaf (1% and 1.2%) and seed (2.46% and 2.9%) essential oil contents were recorded for the Abarkuh population. For ISSR analysis, 15 primer combinations were employed that produced 120 polymorphic bands. Dendrogram and STRUCTURE software grouped the accessions into four clusters although such grouping did not fit the geographic regions perfectly. Among the populations, Abarkuh and Kerman exhibited the highest genetic distance. Based on analysis of molecular variance (AMOVA), only 4.32% of the total genetic diversity was observed among the populations, while 95.68% was detected within the populations. Moreover, the studied populations exhibited a low genetic differentiation (Gst = 0.13) but a high gene flow (Nm = 3.26). It may be concluded that the results of the study provide new insights regarding the genetic diversity of Moshgak germplasm that will be useful for its conservation management and breeding programs for oil- and yield-related traits.
土木香是伞形科的一种芳香蔬菜和药用植物。本研究采用形态学和精油研究以及 ISSR 分析方法，调查了 24 个伊朗种群的 120 个 Moshgak 个体的遗传多样性。在连续两年的农业试验中，观察到种群在形态生理特征（形态和精油含量）方面存在高度变异。在这两年中，Abarkuh 种群的叶片（1%和 1.2%）和种子（2.46%和 2.9%）精油含量最高。对于 ISSR 分析，使用了 15 个引物组合，产生了 120 个多态性条带。尽管这种分组与地理区域不完全吻合，但聚类图和 STRUCTURE 软件将个体分为四个聚类。在种群中，Abarkuh 和克尔曼的遗传距离最高。基于分子方差分析（AMOVA），种群间仅观察到总遗传多样性的 4.32%，而 95.68%存在于种群内。此外，研究种群表现出较低的遗传分化（Gst=0.13）和较高的基因流（Nm=3.26）。可以得出结论，研究结果为土木香种质资源的遗传多样性提供了新的见解，这将有助于其保护管理和与油和产量相关的性状的育种计划。
