Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain.
J Exp Bot. 2010 Mar;61(5):1537-46. doi: 10.1093/jxb/erq022. Epub 2010 Feb 17.
In plant organs and tissues, the neutral storage lipids are confined to discrete spherical organelles called oil bodies. Oil bodies from plant seeds contain 0.6-3% proteins, including oleosins, steroleosins, and caleosins. In this study, a caleosin isoform of approximately 30 kDa was identified in the olive pollen grain. The protein was mainly located at the boundaries of the oil bodies in the cytoplasm of the pollen grain and the pollen tube. In addition, caleosins were also visualized in the cytoplasm at the subapical zone, as well as in the tonoplast of vacuoles present in the pollen tube cytoplasm. The cellular behaviour of lipid bodies in the olive pollen was also monitored during in vitro germination. The number of oil bodies decreased 20-fold in the pollen grain during germination, whereas the opposite tendency occurred in the pollen tube, suggesting that oil bodies moved from one to the other. The data suggest that this pollen caleosin might have a role in the mobilization of oil bodies as well as in the reorganization of membrane compartments during pollen in vitro germination.
在植物器官和组织中，中性储存脂质局限于称为油体的离散球形细胞器。植物种子的油体含有 0.6-3%的蛋白质，包括油体蛋白、甾醇体蛋白和钙蛋白。在这项研究中，在橄榄花粉粒中鉴定出一种约 30 kDa 的钙蛋白同工型。该蛋白主要位于花粉粒和花粉管细胞质中油体的边界处。此外，在花粉管细胞质中的亚顶区以及在花粉管细胞质中存在的液泡的液泡膜上也可以观察到钙蛋白。还监测了橄榄花粉中脂质体在体外萌发过程中的细胞行为。在萌发过程中，花粉粒中的油体数量减少了 20 倍，而花粉管中则出现相反的趋势，这表明油体从一个转移到另一个。数据表明，这种花粉钙蛋白可能在油体的动员以及花粉体外萌发过程中膜隔室的重组中发挥作用。
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