Kulthanaamondhita Promphakkon, Kornsuthisopon Chatvadee, Chansaenroj Ajjima, Trachoo Vorapat, Manokawinchoke Jeeranan, Samaranayake Lakshman, Srithanyarat Supreda Suphanantachat, Osathanon Thanaphum
Center of Excellence for Dental Stem Cell Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
BDJ Open. 2024 Jun 5;10(1):45. doi: 10.1038/s41405-024-00232-5.
The study explored the expression profile of miRNAs in Notch-activated periodontal ligament stem cells (PDLSCs) and examined their potential cellular targets.
PDLSCs were cultured and treated with indirect immobilized Jagged1. The miRNA expression profile was examined using NanoString analysis. Bioinformatic analysis was performed together with enrichment, and miRNA expression was evaluated and validated using a quantitative polymerase chain reaction (qPCR).
A total of 26 miRNAs were differentially expressed in Jagged1 treated PDLSCs compared with the controls. Pathway analysis revealed that altered miRNAs were significantly associated with the transforming growth factor β (TGF-β) signaling pathway. Target prediction analysis demonstrated that 11,170 genes as predictable targets of these altered miRNAs. Enrichment of predicted target genes revealed that they were related to ErbB, Ras and MAPK signaling pathways and small GTPase transduction.
The research concludes that several miRNAs are differentially expressed in jagged-1 treated PDLSCs. In translational terms the differential functionality of these miRNAs offer promise for the development of targeted regenerative materials that are necessary for managing lost tissue replacement in periodontal diseases.
本研究探讨了微小RNA（miRNA）在Notch激活的牙周膜干细胞（PDLSCs）中的表达谱，并检测了它们潜在的细胞靶点。
培养PDLSCs并用间接固定的Jagged1进行处理。使用NanoString分析检测miRNA表达谱。进行生物信息学分析以及富集分析，并使用定量聚合酶链反应（qPCR）评估和验证miRNA表达。
与对照组相比，共有26种miRNA在Jagged1处理的PDLSCs中差异表达。通路分析显示，改变的miRNA与转化生长因子β（TGF-β）信号通路显著相关。靶标预测分析表明，有11170个基因是这些改变的miRNA的可预测靶标。预测靶基因的富集分析显示，它们与表皮生长因子受体（ErbB）、Ras和丝裂原活化蛋白激酶（MAPK）信号通路以及小GTP酶转导有关。
本研究得出结论，几种miRNA在Jagged1处理的PDLSCs中差异表达。从转化医学角度来看，这些miRNA的不同功能为开发靶向再生材料带来了希望，而靶向再生材料对于治疗牙周疾病中组织缺失的替代是必不可少的。
