Hennessy Rosanna C, Dichmann Søs I, Martens Helle Juel, Zervas Athanasios, Stougaard Peter
Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Denmark.
Present address: HJM: University of Copenhagen, Department of Geosciences and Natural Resource Management, Nørregade 10, 1165 København, Denmark.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4204-4211. doi: 10.1099/ijsem.0.004270.
A novel bacterial strain, S40, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated , , and sequences) showed that strain S40 was affiliated with the genus and with PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26 %; DNA-DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40 and PRI-2C genomes displayed lower similarities when compared to all other strains (ANI ≤94.34 %; dDDH ≤57.6 % relatedness). The DNA G+C content of strain S40 was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10-37 °C (optimum, 25-30 °C) and at pH 6-9 (optimum, pH 6-7). Major fatty acids were C (27.9 %), summed feature (C 6/C ω7; 18.0 %) and C cyclo (15.1 %). The respiratory quinone was determined to be Q8 (94 %) and MK8 (95 %) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S40 (=LMG 31467=NCIMB 15235). In addition, we propose that PRI-2C is reclassified and transferred to the species as PRI-2C.
从丹麦西兰岛采集的青薯根际土壤中分离出一株具有强抗真菌活性的新型细菌菌株S40。采用表型、系统发育和基因组相结合的多相分析方法对S40进行特征描述。基于16S rRNA基因和多位点序列分析（串联的 、 、 和 序列）的系统发育分析表明，菌株S40隶属于 属，与PRI-2C为最密切相关菌株[平均核苷酸同一性（ANI），99.26%；DNA-DNA杂交（dDDH），99.20%]。然而，全基因组序列分析显示，与所有其他 菌株相比，S40和PRI-2C的基因组显示出较低的相似性（ANI≤94.34%；dDDH≤57.6%相关性）。菌株S40的DNA G+C含量测定为55.9 mol%。该菌株细胞为革兰氏阴性、杆状、兼性厌氧，在10-37°C（最适温度为25-30°C）和pH 6-9（最适pH为6-7）条件下生长。主要脂肪酸为C （27.9%）、总和特征（C 6/C ω7；18.0%）和C 环（15.1%）。呼吸醌测定为Q8（94%）和MK8（95%），主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。表型、系统发育和基因组分析结果支持以下假设：菌株S40代表 属的一个新物种，为此提出新名称 sp. nov.。模式菌株为S40（=LMG 31467=NCIMB 15235）。此外，我们建议将PRI-2C重新分类并转移到 种，命名为 PRI-2C。
