Bech Pernille Kjersgaard, Schultz-Johansen Mikkel, Glaring Mikkel Andreas, Barbeyron Tristan, Czjzek Mirjam, Stougaard Peter
Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
UPMC University Paris 6, UMR 7139 Marine Plants and Biomolecules, Station Biologique de Roscoff, F-29682 Roscoff, Bretagne, France.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2242-2247. doi: 10.1099/ijsem.0.001933. Epub 2017 Jul 3.
A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark. Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T. The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10-25 °C (optimum 20-25 °C) and at pH 7-9 (optimum pH 7.5). Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources. Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c. The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was determined to be 42.2 mol%. Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria. The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively. Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp. nov. is proposed. The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
从丹麦西兰岛海岸线采集的大叶藻中分离出一株新型细菌菌株S66T。采用多相分析方法，包括表型、系统发育和基因组方法，对菌株S66T进行特征描述。该菌株革兰氏染色阴性，呈杆状，好氧，在10-25°C（最适温度20-25°C）和pH 7-9（最适pH 7.5）条件下生长。此外，菌株S66T能够以海藻多糖琼脂、琼脂糖、紫菜聚糖、κ-卡拉胶、藻酸盐和海带多糖作为唯一碳源生长。主要脂肪酸为C16 : 0、C16 : 1ω7c和C18 : 1ω7c。测定呼吸醌为Q-8，主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。DNA G+C含量测定为42.2 mol%。基于16S rRNA基因和GyrB序列比较的系统发育分析表明，该细菌隶属于γ-变形菌纲交替单胞菌科的副嗜冷杆菌属。菌株S66T的16S rRNA基因和GyrB序列与副嗜冷杆菌属其他成员的相似性百分比分别为94-95%和84-85%。基于S66T的基因组序列，菌株S66T与副嗜冷杆菌属其他成员之间的平均核苷酸同一性（ANI）为77-80%，DNA-DNA杂交预测显示S66T与副嗜冷杆菌属其他物种之间的相关性值均低于24%。表型、系统发育和基因组分析支持了菌株S66T代表副嗜冷杆菌属一个新物种的假设，为此提出了水解副嗜冷杆菌（Paraglaciecola hydrolytica sp. nov.）这一名称。模式菌株为S66T（=LMG 29457T=NCIMB 15060T=DSM 102834T）。
