Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, 1400 Vienna, Austria - Institut de Recherche Agricole pour le Développement (IRAD), PO Box 2123, Yaoundé, Cameroun.
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, 1400 Vienna, Austria - Institut de Recherche en Sciences de la Santé/Direction Régionale de l'Ouest (IRSS/DRO), 01 PO Box 545, Bobo-Dioulasso, Burkina Faso.
Parasite. 2020;27:43. doi: 10.1051/parasite/2020041. Epub 2020 Jun 18.
The production of a large number of mosquitoes of high biological qualities and reliable sex sorting before release are key challenges when applying the sterile insect technique as part of an area-wide integrated pest management approach. There is a need to fully evaluate the production capacity of the equipment developed in order to plan and maintain a daily production level for large-scale operational release activities. This study aimed to evaluate the potential use of the FAO/IAEA larval rearing unit for Aedes aegypti and the subsequent female contamination rate after sex sorting with a Fay-Morlan glass separator. Trays from each rack were tilted and their contents sorted either for each individual tray or after mixing the content of all trays from the rack. The pupal production and the female contamination rate were estimated with respect to day of collection, position of the tray, type of pupae collection, and sorting operator. Results showed significant daily variability of pupal production and female contamination rate, with a high male pupal production level achieved on the second day of collection and estimated female contamination of male pupae reached around 1%. Neither tray position nor type of pupae collection affected the pupal production and female contamination rate. However, the operator had a significant effect on the female contamination rate. These results highlight the need to optimize pupal production at early days of collection and to develop a more effective and automated method of sex separation.
大量具有高生物学质量且可靠的性别分拣的蚊子的生产，是在区域综合虫害管理方法中应用不育昆虫技术的关键挑战。需要充分评估所开发设备的生产能力，以便规划和维持大规模作业释放活动的日常生产水平。本研究旨在评估 FAO/IAEA 幼虫饲养装置在埃及伊蚊中的潜在应用，以及随后使用 Fay-Morlan 玻璃分离器进行性别分拣后的雌性污染率。从每个架子上取下托盘并倾斜，然后按照每个单独托盘或混合架子上所有托盘的内容进行分类。根据收集日、托盘位置、蛹收集类型和分拣操作员来估算蛹的产量和雌性污染率。结果表明，蛹的产量和雌性污染率存在显著的日变化，在收集的第二天达到了较高的雄性蛹产量水平，估计雄性蛹的雌性污染率达到了 1%左右。托盘位置和蛹收集类型均未影响蛹的产量和雌性污染率。然而，操作人员对雌性污染率有显著影响。这些结果强调了需要在收集的早期优化蛹的产量，并开发更有效和自动化的性别分离方法。
