Department of Chemistry, Tianjin Key Laboratory of Molecular Optoelectronic Sciences School of Science, Tianjin University, Tianjin, 300072, China.
Institute for Advanced Interdisciplinary Research University of Jinan, Jinan, 250011, China.
Biosens Bioelectron. 2020 Sep 15;164:112251. doi: 10.1016/j.bios.2020.112251. Epub 2020 May 6.
The rise of mortality rate caused by hepatocellular carcinoma accelerates requirements of biosensors for early liver cancer diagnosis and treatment to improve the clinical prognosis and prolong the survival of patients. However, how to realize label-free, low-cost, easy and fast-detection is the major challenge in the design of biosensors. Water-gated organic field-effect transistors efficiently bridge the gap between semiconductor devices and biological systems, leading to an organic device suitable for health or body signal monitoring. Herein, a kind of high-performance water-gated organic field-effect transistor is developed through the optimization process. This method provides a label-free general sensing platform for the determination of liver cancer biomarker alpha-fetoprotein in 45 minutes, much quicker than traditional methods such as the enzyme-linked immunosorbent assay for several hours. In addition, with the detection limit lower than the cut-off value as well as the ability to achieve quantitative detection, this novel water-gated organic field-effect transistor enables a much broader analysis of other biomarkers in cancer patient samples.
肝癌死亡率的上升加速了用于早期肝癌诊断和治疗的生物传感器的需求，以改善临床预后并延长患者的生存期。然而，如何实现无标记、低成本、简便和快速检测是生物传感器设计中的主要挑战。水门控有机场效应晶体管有效地弥合了半导体器件和生物系统之间的差距，从而产生了适合健康或身体信号监测的有机器件。在此，通过优化过程开发了一种高性能的水门控有机场效应晶体管。该方法为在 45 分钟内测定肝癌标志物甲胎蛋白提供了一种无标记的通用传感平台，比传统方法（如几个小时的酶联免疫吸附测定）快得多。此外，由于检测限低于截止值以及实现定量检测的能力，这种新型水门控有机场效应晶体管能够更广泛地分析癌症患者样本中的其他生物标志物。
