Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.
Joint School of National University of Singapore and Tianjin University, International Campus of Tianjin University, Binhai New City, Fuzhou 350207, China.
Anal Chem. 2022 May 3;94(17):6615-6620. doi: 10.1021/acs.analchem.2c00897. Epub 2022 Apr 21.
The single molecule level determination with a transistor (SiMoT) platform has attracted considerable attention in the recognition of various ultralow abundance biomolecules, while complicated labeling and testing processes limit its further applications. Recently, organic field-effect transistor (OFET)-based biosensors are good candidates for constructing an advanced label-free SiMoT platform due to their facile fabrication process, rapid response time, and low sample volume with a wide range of detection. However, the sensitivity of most OFET-based biosensors is in the order of nM and pM, which cannot meet the detection requirements of ultralow abundance protein. Herein, a label-free SiMoT platform is demonstrated by integrating pillar[]arene as a signal amplifier, and the detection limit can reach 4.75 aM. Besides, by simultaneous determination of α-fetoprotein, carcinoembryonic antigen, and prostate antigen, the proposed multiplexed OFET-based SiMoT platform provides a key step in reliable early cancer diagnosis.
基于晶体管（SiMoT）平台的单分子水平测定在各种超低丰度生物分子的识别中引起了相当大的关注，但其复杂的标记和测试过程限制了其进一步的应用。最近，基于有机场效应晶体管（OFET）的生物传感器由于其易于制造过程、快速响应时间和低样品量以及广泛的检测范围，是构建先进的无标记 SiMoT 平台的良好候选者。然而，大多数基于 OFET 的生物传感器的灵敏度在 nM 和 pM 范围内，无法满足超低丰度蛋白质的检测要求。在此，通过集成杯芳烃作为信号放大器，展示了一种无标记的 SiMoT 平台，检测限可达到 4.75 aM。此外，通过同时测定α-胎蛋白、癌胚抗原和前列腺抗原，所提出的基于多重 OFET 的 SiMoT 平台为可靠的早期癌症诊断提供了关键步骤。
