Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
Faculty of Chemical Engineering and Technology, Cracow University of Technology, Cracow, Poland.
Int J Pharm. 2020 Jul 30;585:119549. doi: 10.1016/j.ijpharm.2020.119549. Epub 2020 Jun 15.
Silver nanoparticles have been used in a range of applications and although they are already employed in medicine, there are new, promising possibilities for their utilization. We investigated the potential of silver nanoparticles obtained with the use of blackcurrant extract in vitro in the LPS-stimulated RAW264.7 macrophages and in vivo in the murine DSS-induced colitis model. The examined formulations contained particles of 95 nm (Ag95) and 213 nm (Ag213) diameter. In vitro, both formulations inhibited nitric oxide (NO) release. In vivo, the preparations alleviated colitis as evidenced by a decreased macroscopic score and myeloperoxidase activity (indicative of neutrophil infiltration). In both cases, the nanoparticles of larger diameter showed better anti-inflammatory properties. Although further tests are required, our results indicate a plausible new use of silver nanoparticles in inflammatory bowel diseases.
银纳米粒子已被应用于多个领域，尽管它们已经被应用于医学领域，但它们的应用仍然存在新的、有前途的可能性。我们研究了使用黑加仑提取物在体外 LPS 刺激的 RAW264.7 巨噬细胞中和体内 DSS 诱导的结肠炎模型中获得的银纳米粒子的潜力。所研究的制剂包含直径为 95nm（Ag95）和 213nm（Ag213）的颗粒。在体外，两种制剂均抑制一氧化氮（NO）的释放。在体内，制剂减轻了结肠炎，表现在宏观评分和髓过氧化物酶活性（指示中性粒细胞浸润）降低。在这两种情况下，直径较大的纳米颗粒表现出更好的抗炎特性。尽管还需要进一步的测试，但我们的结果表明，银纳米粒子在炎症性肠病中具有合理的新用途。
