Jeon Yoo-Na, Ryu Su-Ji, Lee Ha-Yeon, Kim Jang-Oh, Baek Jong-Suep
Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Republic of Korea.
Department of Radiological Science, Kangwon National University, Samcheok 25949, Republic of Korea.
Antibiotics (Basel). 2024 Jul 24;13(8):686. doi: 10.3390/antibiotics13080686.
Synthesis of silver nanoparticles (AgNPs) using plant extracts has been proposed as a more advantageous and environmentally friendly alternative compared to existing physical/chemical methods. In this study, AgNPs were synthesized from silver nitrate using black mulberry (BM) extract. The biosynthesized AgNPs were characterized through an UV-visible spectrometer, X-ray diffraction, and transmission electron microscopy. Additionally, BM-AgNPs were subjected to antioxidant, antibacterial, anti-inflammatory, and anticancer activities. AgNPs biosynthesized from BM extract were dark brown in color and showed a strong peak at 437 nm, confirming that AgNPs were successfully synthesized. The size of AgNPs was 170.17 ± 12.65 nm, the polydispersity index was 0.281 ± 0.07, and the zeta potential value was -56.6 ± 0.56 mV, indicating that the particles were stable. The higher total phenol, flavonoid, and anthocyanin content of BM-AgNPs compared to BM extract indicates that the particles contain multiple active substances due to the formation of AgNPs. The DPPH and ABTS assays showed decreased IC values compared to BM extract, demonstrating improved antioxidant activity. AgNPs inhibited the growth of and at 600 μg/mL, with minimum bactericidal concentrations determined to be 1000 and 1200 μg/mL, respectively. The anti-inflammatory activity was 64.28% at a BM-AgNPs concentration of 250 μg/mL. As the concentration increased, the difference from the standard decreased, indicating the inhibitory effect of AgNPs on bovine serum albumin denaturation. The viability of MCF-7 cells treated with BM-AgNPs was found to be significantly lower than that of cells treated with BM extract. The IC value of BM-AgNPs was determined to be 96.9 μg/mL. This study showed that BM-AgNPs have the potential to be used in the pharmaceutical industry as antioxidant, antibacterial, anti-inflammatory, and anticancer agents.
与现有的物理/化学方法相比，利用植物提取物合成银纳米颗粒（AgNPs）被认为是一种更具优势且环保的替代方法。在本研究中，使用黑桑（BM）提取物从硝酸银合成了AgNPs。通过紫外可见光谱仪、X射线衍射和透射电子显微镜对生物合成的AgNPs进行了表征。此外，对BM-AgNPs进行了抗氧化、抗菌、抗炎和抗癌活性测试。由BM提取物生物合成的AgNPs呈深棕色，在437nm处有一个强峰，证实AgNPs已成功合成。AgNPs的尺寸为170.17±12.65nm，多分散指数为0.281±0.07，zeta电位值为-56.6±0.56mV，表明颗粒是稳定的。与BM提取物相比，BM-AgNPs中总酚、黄酮和花青素含量更高，表明由于AgNPs的形成，颗粒含有多种活性物质。DPPH和ABTS测定显示，与BM提取物相比，IC值降低，表明抗氧化活性提高。AgNPs在600μg/mL时抑制了[具体细菌名称1]和[具体细菌名称2]的生长，最低杀菌浓度分别确定为1000和1200μg/mL。在BM-AgNPs浓度为250μg/mL时，抗炎活性为64.28%。随着浓度增加，与标准的差异减小，表明AgNPs对牛血清白蛋白变性有抑制作用。发现用BM-AgNPs处理的MCF-7细胞的活力明显低于用BM提取物处理的细胞。BM-AgNPs的IC值确定为96.9μg/mL。本研究表明，BM-AgNPs有潜力作为抗氧化、抗菌、抗炎和抗癌剂用于制药工业。
