Udeabor Samuel Ebele, Herrera-Vizcaíno Carlos, Sader Robert, Kirkpatrick C James, Al-Maawi Sarah, Ghanaati Shahram
Department for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, Germany.
Department of Oral and Maxillofacial Surgery, College of Dentistry, King Khalid University, Abha 61471, Saudi Arabia.
Materials (Basel). 2020 Jun 16;13(12):2730. doi: 10.3390/ma13122730.
The permeability and inflammatory tissue reaction to Mucomaix matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect, 12 Wister rats had MM implanted subcutaneously, whereas another 12 rats (control) were sham-operated without biomaterial implantation. On days 3, 15 and 30, explantation was completed (four rats per time-point) to evaluate the tissue reactions to the matrix. Data collected were statistically analyzed using analysis of variance (ANOVA) and Tukey multiple comparisons tests (GraphPad Prism 8). The matrix absorbed the liquid PRF in the ex vivo study. Day 3 post-implantation revealed mild tissue inflammatory reaction with presence of mononuclear cells in the implantation site and on the biomaterial surface (mostly CD68-positive macrophages). The control group at this stage had more mononuclear cells than the test group. From day 15, multinucleated giant cells (MNGCs) were seen in the implantation site and the outer third of the matrix with marked increase on day 30 and spread to the matrix core. The presence of these CD68-positive MNGCs was associated with significant matrix vascularization. The matrix degraded significantly over the study period, but its core was still visible as of day 30 post-implantation. The high permeability and fast degradation properties of MM were highlighted.
对一种非交联胶原蛋白基基质Mucomaix基质（MM）的渗透性和炎症组织反应进行了体外和体内评估。富含血小板的液态纤维蛋白（PRF），一种血液浓缩系统，用于评估其体外吸收人类蛋白质和与血细胞相互作用的能力。在体内方面，将MM皮下植入12只Wistar大鼠，而另外12只大鼠（对照组）进行假手术，不植入生物材料。在第3、15和30天，完成组织外植（每个时间点4只大鼠）以评估组织对基质的反应。收集的数据使用方差分析（ANOVA）和Tukey多重比较检验（GraphPad Prism 8）进行统计分析。在体外研究中，该基质吸收了液态PRF。植入后第3天，植入部位和生物材料表面出现轻度组织炎症反应，有单核细胞存在（大多为CD68阳性巨噬细胞）。此时对照组的单核细胞比试验组更多。从第15天起，在植入部位和基质的外三分之一处可见多核巨细胞（MNGC），在第30天显著增加并扩散至基质核心。这些CD68阳性MNGC的存在与基质显著血管化相关。在研究期间，该基质显著降解，但在植入后第30天其核心仍可见。突出了MM的高渗透性和快速降解特性。
