Baldassarre Francesca, Tatulli Giuseppe, Vergaro Viviana, Mariano Stefania, Scala Valeria, Nobile Concetta, Pucci Nicoletta, Dini Luciana, Loreti Stefania, Ciccarella Giuseppe
Biological and Environmental Sciences Department, UdR INSTM of Lecce University of Salento, Via Monteroni, 73100 Lecce, Italy.
Institute of Nanotechnology, CNR NANOTEC, Consiglio Nazionale delle Ricerche, Via Monteroni, 73100 Lecce, Italy.
Nanomaterials (Basel). 2020 Jun 16;10(6):1174. doi: 10.3390/nano10061174.
Recently, there is a growing demand in sustainable phytopathogens control research. Nanotechnology provides several tools such as new pesticides formulations, antibacterial nanomaterials and smart delivery systems. Metal nano-oxides and different biopolymers have been exploited in order to develop nanopesticides which can offer a targeted solution minimizing side effects on environment and human health. This work proposed a nanotechnological approach to obtain a new formulation of systemic fungicide fosetyl-Al employing ultrasonication assisted production of water dispersible nanocrystals. Moreover, chitosan was applicated as a coating agent aiming a synergistic antimicrobial effect between biopolymer and fungicide. Fosetyl-Al nanocrystals have been characterized by morphological and physical-chemical analysis. Nanotoxicological investigation was carried out on human keratinocytes cells through cells viability test and ultrastructural analysis. planktonic growth, biofilm production and agar dilution assays have been conducted on two subspecies. Fosetyl-Al nanocrystals resulted very stable over time and less toxic respect to conventional formulation. Finally, chitosan-based fosetyl-Al nanocrystals showed an interesting antibacterial activity against subsp. and subsp.
最近，可持续植物病原体控制研究的需求日益增长。纳米技术提供了多种工具，如新型农药配方、抗菌纳米材料和智能递送系统。金属纳米氧化物和不同的生物聚合物已被用于开发纳米农药，这种纳米农药可以提供有针对性的解决方案，最大限度地减少对环境和人类健康的副作用。这项工作提出了一种纳米技术方法，通过超声辅助生产水分散性纳米晶体来获得新型系统性杀菌剂乙膦铝的配方。此外，壳聚糖被用作包衣剂，旨在实现生物聚合物与杀菌剂之间的协同抗菌作用。通过形态学和物理化学分析对乙膦铝纳米晶体进行了表征。通过细胞活力测试和超微结构分析对人角质形成细胞进行了纳米毒理学研究。对两个亚种进行了浮游生长、生物膜形成和琼脂稀释试验。乙膦铝纳米晶体随时间推移非常稳定，且相对于传统配方毒性更小。最后，基于壳聚糖的乙膦铝纳米晶体对亚种和亚种显示出有趣的抗菌活性。
