Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119991 8-2 Trubetskaya St., Moscow, Russia; FSBSI Institute of General Pathology and Pathophysiology, 125315, 8 Baltiyskaya St., Moscow, Russia; FSBEI FPE "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of Russia, 125993, 2/1-1 Barrikadnaya St., Moscow, Russia.
Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119991 8-2 Trubetskaya St., Moscow, Russia.
Acta Biomater. 2020 Sep 1;113:63-83. doi: 10.1016/j.actbio.2020.06.016. Epub 2020 Jun 17.
Cell sheet technology has remained quite popular among tissue engineering techniques over the last several years. Meanwhile, there is an apparent trend in modern scientific research towards combining different approaches and strategies. Accordingly, a large body of work has arisen where cell sheets are used not as separate structures, but in combination with scaffolds as supporting constructions. The aim of this review is to analyze the intersection of these two vast areas of tissue engineering described in the literature mainly within the last five years. Some practical and technical details are emphasized to provide information that can be useful in research design and planning. The first part of the paper describes the general issues concerning the use of combined technology, its advantages and limitations in comparison with those of other tissue engineering approaches. Next, the detailed literature analysis of in vivo studies aimed at the regeneration of different tissues is performed. A significant part of this section concerns bone regeneration. In addition to that, other connective tissue structures, including articular cartilage and fibrocartilage, ligaments and tendons, and some soft tissues are discussed. STATEMENT OF SIGNIFICANCE: This paper describes the intersection of two technologies used in designing of tissue-engineered constructions for regenerative medicine: cell sheets as extracellular matrix-rich structures and supporting scaffolds as essentials in tissue engineering. A large number of reviews are devoted to each of these scientific problems. However, the solution of complex problems of tissue engineering requires an integrated approach that includes both three-dimensional scaffolds and cell sheets. This manuscript serves as a description of advantages and limitations of this method, its use in regeneration of bones, connective tissues and soft tissues and some other details.
细胞片技术在过去几年中一直是组织工程技术中非常流行的技术。与此同时，现代科学研究明显呈现出结合不同方法和策略的趋势。因此，大量的工作已经出现，其中细胞片不再作为单独的结构使用，而是与支架一起作为支撑结构使用。本文的目的是分析文献中主要在过去五年内描述的这两个广泛的组织工程领域的交叉点。强调了一些实际和技术细节，以提供在研究设计和规划中可能有用的信息。本文的第一部分描述了使用组合技术的一般问题，以及与其他组织工程方法相比，它的优点和局限性。接下来，对旨在再生不同组织的体内研究的详细文献分析进行了执行。这一部分的很大一部分涉及骨再生。除此之外，还讨论了其他结缔组织结构，包括关节软骨和纤维软骨、韧带和肌腱以及一些软组织。意义声明：本文描述了两种用于设计再生医学组织工程结构的技术的交叉点：富含细胞外基质的细胞片和作为组织工程基础的支撑支架。大量的评论都致力于解决这些科学问题中的每一个。然而，解决组织工程的复杂问题需要一种综合的方法，包括三维支架和细胞片。本文档旨在描述这种方法的优缺点，其在骨骼、结缔组织和软组织以及其他一些细节的再生中的应用。
