Holstad G, Teige J
Acta Vet Scand. 1988;29(3-4):281-6. doi: 10.1186/BF03548619.
Twelve kids, 5 1/2 months old, were inoculated intravenously with about 1 million colony forming units of a Corynebacterium pseudotuberculosis strain isolated from goat. Nine of the animals had antibodies against the organism in the bacterial agglutination test and/or the hemolysis inhibition test before they were inoculated. Four kids developed acute toxemia and died 2–5 days after the inoculation. Three of these animals were negative in both the bacterial agglutination test and the hemolysis inhibition test, while the fourth was positive in the bacterial agglutination test only. Post mortem examination revealed severe icterus, anemia, hemoglobinuria and acute pneumonia with microabscess formation in 3 of the kids that died. Eight animals, all with antibodies against C. pseudotuberculosis, developed acute illness but survived the inoculation. These animals were sacrificed and examined post mortem 1 month after the experimental infection, and abscesses were demonstrated in internal organs in all cases. It is concluded that intravenous inoculation with C. pseudotuberculosis is not a suitable challenge system to study the prophylactic efficacy of vaccines against caseous lymphadenitis in goats.
给12只5个半月大的小羊静脉注射了约100万个从山羊分离出的伪结核棒状杆菌菌株的菌落形成单位。其中9只动物在接种前的细菌凝集试验和/或溶血抑制试验中就已具有针对该菌的抗体。4只小羊在接种后2至5天出现急性毒血症并死亡。其中3只动物在细菌凝集试验和溶血抑制试验中均为阴性，而第4只仅在细菌凝集试验中呈阳性。尸检发现，死亡的3只小羊出现严重黄疸、贫血、血红蛋白尿和伴有微脓肿形成的急性肺炎。8只均具有抗伪结核棒状杆菌抗体的动物出现急性病症，但接种后存活。在实验感染1个月后，对这些动物实施安乐死并进行尸检，结果发现所有动物的内脏均有脓肿。得出的结论是，静脉注射伪结核棒状杆菌并非研究疫苗对山羊干酪性淋巴结炎预防效果的合适攻毒系统。
