Juthani Nidhi, Doyle Patrick S
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Analyst. 2020 Aug 7;145(15):5134-5140. doi: 10.1039/d0an00938e. Epub 2020 Jun 22.
We report a platform utilizing a reporter enzyme, which produces a chromogenic indigo precipitate that preferentially localizes within a hydrogel microparticle. The 3D network of the hydrogel maintains the rapid target binding kinetics found in solution, while multiplexed target detection is achieved through shape-encoding of the particles. Moreover, the precipitate-laden hydrogels can be imaged with a simple phone camera setup. We used this system to detect microRNA (miRNA) down to 0.22 fmol. We then showed the compatibility of this system with real samples by performing multiplexed miRNA measurements from total RNA from matched colon cancer and normal adjacent tissue.
我们报道了一种利用报告酶的平台，该酶可产生一种发色靛蓝沉淀，该沉淀优先定位于水凝胶微粒内。水凝胶的三维网络保持了溶液中快速的靶标结合动力学，同时通过微粒的形状编码实现了多重靶标检测。此外，负载沉淀的水凝胶可以用简单的手机摄像头装置成像。我们使用该系统检测低至0.22飞摩尔的微小RNA（miRNA）。然后，我们通过对匹配的结肠癌和正常相邻组织的总RNA进行多重miRNA测量，展示了该系统与实际样本的兼容性。
