Mo Kun-Lian, Wang Ling, Wu Qing-Juan, Ye Lin, Liu Xing-di, Hu Yong-Hua, Huang Hui-Qin
Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources, Haikou 571101, PR China.
Institute of Tropical Bioscience and Biotechnology, Hainan Academy of Tropical Agricultural Resource, CATAS, Haikou 571101, PR China.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4245-4249. doi: 10.1099/ijsem.0.004280.
A Gram-stain-negative, short-rod-shaped and pink-pigmented bacterial strain (HB172049) was isolated from mangrove sediment. Cells grew at 10-45 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, pH 7.0) and with 0.5-9.0 % (w/v) NaCl (optimum, 2-5 %). Analysis of the 16S rRNA gene sequence revealed that the isolate had highest sequence similarities to DSM 100162 (96.5 %) and X14-1 (96.5 %). The values of average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization between the isolate and its close neighbours were, respectively, less than 80.1, 81.7 and 23.2 %. Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the predominant cellular fatty acids were summed feature 4 and iso-C (42.2 and 24.6 %, respectively). The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified polar lipids. The genomic DNA G+C content was 52.6 mol%. Based on polyphasic taxonomic characterization, it is proposed that strain HB172049 belongs to the genus and represents a novel species, for which the name sp. nov. is proposed. The type strain is HB172049 (=CGMCC 1.16729=JCM 33333).
从红树林沉积物中分离出一株革兰氏阴性、短杆状且呈粉红色的细菌菌株（HB172049）。细胞在10 - 45°C（最适温度为30°C）、pH 6.0 - 9.0（最适pH为7.0）以及含有0.5 - 9.0%（w/v）NaCl（最适浓度为2 - 5%）的条件下生长。16S rRNA基因序列分析表明，该分离株与DSM 100162（96.5%）和X14 - 1（96.5%）具有最高的序列相似性。该分离株与其近缘菌株之间的平均核苷酸同一性、平均氨基酸同一性和数字DNA - DNA杂交值分别低于80.1%、81.7%和23.2%。化学分类分析表明，唯一的呼吸醌为MK - 7，主要的细胞脂肪酸为特征性总和4和异-C（分别为42.2%和24.6%）。主要极性脂质包括磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂、一种未鉴定的磷脂、一种未鉴定的氨基磷脂和两种未鉴定的极性脂质。基因组DNA的G + C含量为52.6 mol%。基于多相分类特征，建议将菌株HB172049归为该属，并代表一个新物种，为此提出新物种名称为 sp. nov.。模式菌株为HB172049（=CGMCC 1.16729 =JCM 33333）。
