Park Seona, Lee Hyang Burm, Srinivasan Sathiyaraj, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, South Korea.
Environmental Microbiology Lab, Department of Agricultural Biological Chemistry, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 61186, South Korea.
Int J Syst Evol Microbiol. 2025 Apr;75(4). doi: 10.1099/ijsem.0.006755.
Three novel bacterial strains, 172403-2, BT310 and BT731, were isolated from UV-irradiated soil samples collected in South Korea. All strains are Gram-negative, aerobic, non-motile and grow at 20-35 °C, optimally at 30 °C and pH 7.0. The optimal NaCl concentration for strains 172403-2 and BT310 is 1.5%, while strain BT731 grows optimally in the absence of NaCl (0.0%). Phylogenetic analysis based on 16S rRNA gene sequences positioned these strains within the genus , with strain 172403-2 closest to (95.96%), BT310 to (97.87%) and BT731 to (98.06%). Cellular fatty acid profiles identified C iso and Summed Feature 4 as predominant fatty acids. All strains contained ubiquinone MK-7 and phosphatidylethanolamine as major respiratory quinone and primary polar lipids, respectively. Genome sizes were 5.08 Mb for 172403-2, 4.29 Mb for BT310 and 4.66 Mb for BT731, with average nucleotide identity and digital DNA-DNA hybridization values with other species ranging between 70.41%-88.56% and 11.55%-24.66%, respectively. These biochemical, chemotaxonomic and phylogenetic analyses confirm that strains 172403-2, BT310 and BT731 represent three novel species of , proposed as sp. nov. (172403-2 = KCTC 62072 =NBRC 114967), sp. nov. (BT310 = KCTC 72363 = NBRC 114846) and sp. nov. (BT731 = KCTC 92910 = NBRC 116069).
从韩国采集的紫外线照射土壤样本中分离出三株新型细菌菌株，172403-2、BT310和BT731。所有菌株均为革兰氏阴性、需氧、无运动性，在20-35°C下生长，最适生长温度为30°C，最适pH值为7.0。菌株172403-2和BT310的最佳NaCl浓度为1.5%，而菌株BT731在无NaCl（0.0%）的条件下生长最佳。基于16S rRNA基因序列的系统发育分析将这些菌株定位在该属内，菌株172403-2与[具体菌名1]最接近（95.96%），BT310与[具体菌名2]最接近（97.87%），BT731与[具体菌名3]最接近（98.06%）。细胞脂肪酸谱鉴定出异C和总和特征4为主要脂肪酸。所有菌株分别含有泛醌MK-7和磷脂酰乙醇胺作为主要呼吸醌和主要极性脂质。172403-2的基因组大小为5.08 Mb，BT310为4.29 Mb，BT731为4.66 Mb，与其他[属名]物种的平均核苷酸同一性和数字DNA-DNA杂交值分别在70.41%-88.56%和11.55%-24.66%之间。这些生化、化学分类和系统发育分析证实，菌株172403-2、BT310和BT731代表[属名]的三个新物种，分别命名为[新物种名1] sp. nov.（172403-2 = KCTC 62072 =NBRC 114967）、[新物种名2] sp. nov.（BT310 = KCTC 72363 = NBRC 114846）和[新物种名3] sp. nov.（BT731 = KCTC 92910 = NBRC 116069）。
