Medicinal, Aromatic and Economic Plants Group, Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam 785006, India; AcSIR-Academy of Scientific and Innovative Research, CSIR-North East Institute of Science and Technology, Jorhat, Assam 785006, India.
Medicinal, Aromatic and Economic Plants Group, Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam 785006, India.
Food Chem. 2020 Nov 30;331:127278. doi: 10.1016/j.foodchem.2020.127278. Epub 2020 Jun 10.
H quantitative Nuclear Magnetic Resonance (qNMR) spectroscopy technique has certain advantages such as low-temperature operation, authentic structural prediction and short data acquisition time. In this study, a H qNMR method was developed for the analysis of propenylbenzenes (eugenol and seven analogues) in the essential oils, a broadly distributed class of natural flavours. It was validated in terms of specificity (methoxy/acetate signal), linearity (range 0.05-5.00 mg per assay), sensitivity (limit of detection and quantification 4.4 and 14.9 µg/mL respectively), accuracy and precision. The qNMR technique was utilized during the sensory or activity-guided identification of chavibetol as the key odorant and antioxidant in the betel (Piper betle L., Bangla cultivar) oil, a widely consumed chewing stimulant and valuable flavouring agent. The method was also applied for the evaluation of six different post-harvest drying techniques for betel leaves through the quantitative analysis of unambiguously identified propenylbenzene markers (chavibetol, chavibetol acetate and 4-allyl-1,2-phenylene diacetate).
H 定量核磁共振（qNMR）光谱技术具有低温操作、真实结构预测和短数据采集时间等优点。在这项研究中，开发了一种 H qNMR 方法，用于分析精油中的丙烯基苯（丁香酚和七种类似物），这是一类广泛分布的天然香料。该方法在特异性（甲氧基/乙酸酯信号）、线性范围（0.05-5.00 mg/测定）、灵敏度（检测限和定量限分别为 4.4 和 14.9 µg/mL）、准确性和精密度方面得到了验证。在感官或活性导向鉴定中，qNMR 技术用于鉴定 chavibetol 作为 betel（Piper betle L.，孟加拉品种）油中的关键气味和抗氧化剂，betel 油是一种广泛消费的咀嚼兴奋剂和有价值的调味剂。该方法还通过定量分析明确鉴定的丙烯基苯标志物（chavibetol、chavibetol 乙酸酯和 4-丙烯基-1,2-亚苯基二乙酸酯），用于评估六种不同的收获后干燥技术对 betel 叶的影响。
