Osman Muhamad Faris, Lee Soo Yee, Sarbini Shahrul Razid, Mohd Faudzi Siti Munirah, Khamis Shamsul, Zainudin Badrul Hisyam, Shaari Khozirah
Natural Medicines and Products Research Laboratory (NaturMeds), Institute of Bioscience, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, Malaysia.
Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan 25200, Pahang, Malaysia.
Plants (Basel). 2021 Nov 19;10(11):2510. doi: 10.3390/plants10112510.
The differences in pungency of "sirih" imply the probable occurrence of several variants of L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the H-NMR spectral data showed quantitative differences in the metabolite profiles of "sirih melayu" and "sirih india" and revealed an ambiguous group of samples with low acetic acid content, which was identified as hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into "sirih melayu" and "sirih india" groups. Higher abundance of chavicol acetate was consistently found to be characteristic of "sirih melayu". The present research has provided preliminary evidence supporting the notion of occurrence of two variants in Malaysia based on chemical profiles, which may be related to the different genders of .
“蒌叶”辛辣程度的差异意味着马来西亚可能存在几种蒌叶变种。然而，不同变种辛辣味背后的代谢物谱仍然是进一步研究的课题。因此，对选定的马来西亚变种的代谢物谱差异进行了研究；具体而言，分别通过氢核磁共振（H-NMR）和气相色谱-质谱联用（GC-MS）代谢组学分析了叶片的甲醇水提取物和精油。H-NMR光谱数据的主成分分析（PCA）显示了“马来蒌叶”和“印度蒌叶”代谢物谱的定量差异，并揭示了一组乙酸含量低的模糊样本组，根据内部转录间隔区2（ITS2）区域的DNA序列将其鉴定为hort. ex Rodigas。从马来西亚半岛几个州采集的样本的两个GC-MS数据集的PCA支持了这一发现，该分析将样本聚类为“马来蒌叶”和“印度蒌叶”组。一直发现乙酸丁香酚酯含量较高是“马来蒌叶”的特征。本研究提供了初步证据，支持基于化学特征在马来西亚存在两种变种的观点，这可能与蒌叶的不同性别有关。
