MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Mikrochim Acta. 2023 Nov 1;190(11):455. doi: 10.1007/s00604-023-06037-x.
Helicobacter pylori (H. pylori) is a zoonotic gastric microorganism capable of efficient interspecies transmission. Domesticated companion animals, particularly dogs and cats, serve as natural reservoirs for H. pylori. This phenomenon facilitates the extensive dissemination of H. pylori among households with pets. Hence, the prompt and precise identification of H. pylori in companion animals holds paramount importance for the well-being of both animals and their owners. With the assistance of Multienzyme Isothermal Rapid Amplification (MIRA) and CRISPR-Cas12a system, we successfully crafted a highly adaptable optical detection platform for H. pylori. Three sensor systems with corresponding visual interpretations were proposed. This study demonstrated a rapid turnaround time of approximately 45 min from DNA extraction to the result display. Moreover, this platform topped germiculture and real-time PCR in terms of sensitivity or efficiency in clinical diagnoses of 66 samples. This platform possesses significant potential as a versatile approach and represents the premiere application of CRISPR for the non-invasive detection of H. pylori in companion animals, thereby mitigating the dissemination of H. pylori among household members.
幽门螺杆菌（H. pylori）是一种能够进行有效种间传播的动物源性胃微生物。家养的伴侣动物，特别是狗和猫，是 H. pylori 的天然宿主。这一现象促进了 H. pylori 在有宠物的家庭中的广泛传播。因此，及时准确地识别伴侣动物中的 H. pylori 对动物及其主人的健康都至关重要。借助多酶等温快速扩增（MIRA）和 CRISPR-Cas12a 系统，我们成功地为 H. pylori 构建了一个高度适配的光学检测平台。提出了三个具有相应视觉解释的传感器系统。该研究从 DNA 提取到结果显示，实现了大约 45 分钟的快速周转时间。此外，在对 66 个样本的临床诊断中，该平台在灵敏度或效率方面优于细菌培养和实时 PCR。该平台具有作为一种通用方法的巨大潜力，代表了 CRISPR 在伴侣动物中进行非侵入性检测 H. pylori 的首次应用，从而减少了 H. pylori 在家庭成员中的传播。
