Kim Hyoun Joong, Giri Sib Sankar, Kim Sang Guen, Kim Sang Wha, Kwon Jun, Lee Sung Bin, Park Se Chang
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
Microorganisms. 2020 Jun 19;8(6):926. doi: 10.3390/microorganisms8060926.
is one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and control spp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenic from an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified as and were stable over a wide range of temperatures (4-37 °C) and at pH 7.0-9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenic in an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality against infection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenic infection, but also to reduce antibiotic overuse.
是导致海洋双壳贝类幼虫水产养殖大量死亡的主要病原体之一。为了预防和控制海洋双壳贝类孵化场中的 感染，各种抗生素被过度使用，导致环境污染和产生多重耐药菌株。因此，需要开展抗生素替代品的研发研究。在本研究中，我们从一个牡蛎孵化场分离出两种特异性感染致病性 的噬菌体，并将它们命名为 pVco - 5 和 pVco - 7。这两种噬菌体均被归类为 ，并且在很宽的温度范围（4 - 37°C）和pH值7.0 - 9.0条件下都很稳定。因此，这两种噬菌体都适合在牡蛎孵化场的环境条件下应用。在体外试验中，这两种噬菌体对致病性 显示出确证的显著杀菌效果。在体内实验中，太平洋牡蛎幼虫的噬菌体预处理组在 感染时的死亡率显著低于未处理的对照幼虫。本研究结果表明，这两种噬菌体均可用于人工海水双壳贝类育苗产业；不仅可以预防致病性 感染，还能减少抗生素的过度使用。
