Sekaly R P, Jacobson S, Richert J R, Tonnelle C, McFarland H F, Long E O
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1209-12. doi: 10.1073/pnas.85.4.1209.
A human fibroblast line expressing HLA-DR1 antigen on its surface was generated by transfection with DR alpha and DR beta cDNAs. Expression of the invariant chain gene was not detectable in the transfected fibroblasts and was not induced by infection with measles virus. Lysis of measles virus-infected cells occurred with DR1- but not with DR4-restricted measles virus-specific cytotoxic T-lymphocyte (CTL) clones and was inhibited by a monoclonal antibody specific for DR antigen. Therefore, the invariant chain is not required for DR-restricted presentation of measles virus antigens by this fibroblast line. Transfected fibroblasts were lysed as efficiently as an autologous B-cell line even though they expressed much less surface DR antigen. Lysis of both the transfected fibroblasts and the B-cell line was insensitive to treatment with chloroquine. These results demonstrate that expression of a DR alpha beta heterodimer at the surface of this fibroblast line is necessary and sufficient for presentation of measles virus antigens to specific CTL clones.
通过用DRα和DRβ cDNA转染，产生了一种在其表面表达HLA - DR1抗原的人成纤维细胞系。在转染的成纤维细胞中未检测到恒定链基因的表达，并且麻疹病毒感染也未诱导其表达。麻疹病毒感染细胞的裂解发生在DR1限制而非DR4限制的麻疹病毒特异性细胞毒性T淋巴细胞（CTL）克隆中，并且被针对DR抗原的单克隆抗体所抑制。因此，对于该成纤维细胞系呈递麻疹病毒抗原而言，恒定链并非必需。尽管转染的成纤维细胞表达的表面DR抗原少得多，但它们被裂解的效率与自体B细胞系一样高。转染的成纤维细胞和B细胞系的裂解对氯喹处理均不敏感。这些结果表明，在该成纤维细胞系表面表达DRαβ异二聚体对于将麻疹病毒抗原呈递给特异性CTL克隆是必要且充分的。
