Jacobson S, Sekaly R P, Jacobson C L, McFarland H F, Long E O
Neuroimmunology Branch, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, Maryland 20892.
J Virol. 1989 Apr;63(4):1756-62. doi: 10.1128/JVI.63.4.1756-1762.1989.
To analyze the nature of the HLA class II-restricted cytotoxic T-lymphocyte (CTL) response to measles virus, murine fibroblasts were transfected with expressible cDNA clones for human HLA-DR antigen and for measles virus matrix or nucleocapsid proteins. DR-positive murine fibroblasts transfected with measles virus matrix or nucleocapsid genes were lysed by class II-restricted measles virus-specific CTL lines. Lysis was as efficient as with infected autologous B-cell lines, even though the measles virus cytoplasmic proteins were undetectable by antibodies in the transfected target cells. These results demonstrate that cytoplasmic viral antigens can be presented to CTL in the context of HLA class II antigens and that measles virus matrix and nucleocapsid proteins contribute to class II-restricted measles virus-specific CTL responses. These results also show that endogenously synthesized measles virus proteins can be efficiently presented by class II antigens. The implications of these findings for measles virus pathogenesis and for antigen processing are discussed.
为分析HLA II类分子限制性细胞毒性T淋巴细胞（CTL）对麻疹病毒反应的性质，用人类HLA - DR抗原及麻疹病毒基质或核衣壳蛋白的可表达cDNA克隆转染鼠成纤维细胞。转染了麻疹病毒基质或核衣壳基因的DR阳性鼠成纤维细胞被II类分子限制性麻疹病毒特异性CTL系裂解。裂解效率与感染的自体B细胞系相同，尽管在转染的靶细胞中抗体检测不到麻疹病毒胞质蛋白。这些结果表明，胞质病毒抗原可在HLA II类抗原的背景下呈递给CTL，且麻疹病毒基质和核衣壳蛋白有助于II类分子限制性麻疹病毒特异性CTL反应。这些结果还表明，内源性合成的麻疹病毒蛋白可被II类抗原有效呈递。讨论了这些发现对麻疹病毒发病机制及抗原加工的意义。
