Anastassakos C, Alexander G J, Wolstencroft R A, Avery J A, Portmann B C, Panayi G S, Dumonde D C, Eddleston A L, Williams R
Liver Unit, King's College School of Medicine and Dentistry, London, United Kingdom.
Gastroenterology. 1988 Apr;94(4):999-1005. doi: 10.1016/0016-5085(88)90559-8.
Abnormalities of lymphocyte proliferation in chronic hepatitis B virus infection are well documented, although the underlying mechanisms are poorly understood. To determine whether these defects may be secondary to disordered lymphokine production, we have simultaneously assayed interleukin-1 and interleukin-2 production in 31 chronic carriers of the hepatitis B virus. Supernatants from mononuclear cells cultured both in the presence and absence of lipopolysaccharide contained significantly increased quantities of interleukin-1 activity in patients compared with normal controls (p less than 0.01). Lysates of monocytes from patients also contained more interleukin-1 than those of controls (p less than 0.05) in the presence of lipopolysaccharide or silica, or both. These results indicate that interleukin-1 production is markedly elevated in patients with chronic hepatitis B virus infection, whereas in contrast, interleukin-2 production was found to be reduced in these patients (p less than 0.01). As one of the biological properties of interleukin-1 is to stimulate fibroblasts to produce collagen, the relationship between fibrosis in the liver biopsy specimen and interleukin production was examined. There was a highly significant correlation (p less than 0.001) between interleukin-1 production and the severity of fibrosis, suggesting that this lymphokine may be closely related to the development of cirrhosis in such patients.
慢性乙肝病毒感染中淋巴细胞增殖异常已有充分记录，但其潜在机制尚不清楚。为了确定这些缺陷是否可能继发于淋巴因子产生紊乱，我们同时检测了31例乙肝病毒慢性携带者白细胞介素-1和白细胞介素-2的产生情况。与正常对照相比，在有和无脂多糖存在的情况下培养的单核细胞上清液中，患者的白细胞介素-1活性显著增加（p<0.01）。在有脂多糖或二氧化硅或两者存在的情况下，患者单核细胞裂解物中的白细胞介素-1也比对照更多（p<0.05）。这些结果表明，慢性乙肝病毒感染患者的白细胞介素-1产生明显升高，而相反，这些患者的白细胞介素-2产生减少（p<0.01）。由于白细胞介素-1的生物学特性之一是刺激成纤维细胞产生胶原蛋白，因此研究了肝活检标本中的纤维化与白细胞介素产生之间的关系。白细胞介素-1产生与纤维化严重程度之间存在高度显著的相关性（p<0.001），表明这种淋巴因子可能与此类患者肝硬化的发展密切相关。
