Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, United Kingdom.
ACS Biomater Sci Eng. 2020 Jun 8;6(6):3513-3528. doi: 10.1021/acsbiomaterials.0c00191. Epub 2020 Apr 29.
Angiogenesis assays are essential for studying aspects of neovascularization and angiogenesis and investigating drugs that stimulate or inhibit angiogenesis. To date, there are several and angiogenesis assays that are used for studying different aspects of angiogenesis. Although assays are the most representative of native angiogenesis, they raise ethical questions, require considerable technical skills, and are expensive. assays are inexpensive and easier to perform, but the majority of them are only two-dimensional cell monolayers which lack the physiological relevance of three-dimensional structures. Thus, it is important to look for alternative platforms to study angiogenesis under more physiologically relevant conditions . Accordingly, in this study, we developed polymeric vascular networks to be used to study angiogenesis and vascularization of a 3D human skin model . Our results showed that this platform allowed the study of more than one aspect of angiogenesis, endothelial migration and tube formation, when combined with Matrigel. We successfully reconstructed a human skin model, as a representative of a physiologically relevant and complex structure, and assessed the suitability of the developed platform for studying endothelialization of the tissue-engineered skin model.
血管生成分析对于研究血管新生和血管生成的各个方面以及研究刺激或抑制血管生成的药物至关重要。迄今为止，有几种体内和体外血管生成分析用于研究血管生成的不同方面。虽然体内分析最能代表天然血管生成，但它们存在伦理问题，需要相当高的技术技能，而且成本昂贵。体外分析成本低廉，更容易进行，但它们中的大多数只是二维细胞单层，缺乏三维结构的生理相关性。因此，寻找替代平台来在更接近生理条件下研究血管生成非常重要。因此，在这项研究中，我们开发了聚合物血管网络，用于研究 3D 人皮肤模型中的血管生成和血管化。我们的结果表明，当与 Matrigel 结合使用时，该平台允许研究血管生成的多个方面，包括内皮细胞迁移和管状结构形成。我们成功地重建了人皮肤模型，作为生理相关和复杂结构的代表，并评估了开发的聚合物血管网络平台用于研究组织工程皮肤模型的内皮化的适用性。
