Aldemir Dikici Betül, Dikici Serkan, Reilly Gwendolen C, MacNeil Sheila, Claeyssens Frederik
Department of Materials Science and Engineering, University of Sheffield, Kroto Research Institute, Sheffield S3 7HQ, UK.
Department of Materials Science and Engineering, University of Sheffield, INSIGNEO Institute for in silico Medicine, The Pam Liversidge Building, Sheffield S1 3JD, UK.
Materials (Basel). 2019 Aug 20;12(16):2643. doi: 10.3390/ma12162643.
Guided bone regeneration is a common dental implant treatment where a barrier membrane (BM) is used between epithelial tissue and bone or bone graft to prevent the invasion of the fast-proliferating epithelial cells into the defect site to be able to preserve a space for infiltration of slower-growing bone cells into the periodontal defect site. In this study, a bilayer polycaprolactone (PCL) BM was developed by combining electrospinning and emulsion templating techniques. First, a 250 µm thick polymerised high internal phase emulsion (polyHIPE) made of photocurable PCL was manufactured and treated with air plasma, which was shown to enhance the cellular infiltration. Then, four solvent compositions were investigated to find the best composition for electrospinning a nanofibrous PCL barrier layer on PCL polyHIPE. The biocompatibility and the barrier properties of the electrospun layer were demonstrated over four weeks in vitro by histological staining. Following in vitro assessment of cell viability and cell migration, cell infiltration and the potential of PCL polyHIPE for supporting blood vessel ingrowth were further investigated using an ex-ovo chick chorioallantoic membrane assay. Our results demonstrated that the nanofibrous PCL electrospun layer was capable of limiting cell infiltration for at least four weeks, while PCL polyHIPE supported cell infiltration, calcium and mineral deposition of bone cells, and blood vessel ingrowth through pores.
引导骨再生是一种常见的牙种植治疗方法，即在上皮组织与骨或骨移植材料之间使用屏障膜（BM），以防止快速增殖的上皮细胞侵入缺损部位，从而为生长较慢的骨细胞渗入牙周缺损部位保留空间。在本研究中，通过结合静电纺丝和乳液模板技术制备了一种双层聚己内酯（PCL）屏障膜。首先，制备了由光固化PCL制成的250 µm厚的聚合高内相乳液（polyHIPE），并用空气等离子体处理，结果表明这能增强细胞浸润。然后，研究了四种溶剂组合物，以找到在PCL polyHIPE上静电纺丝纳米纤维PCL屏障层的最佳组合物。通过组织学染色在体外四周内证明了静电纺丝层的生物相容性和屏障性能。在对细胞活力和细胞迁移进行体外评估后，使用鸡胚绒毛尿囊膜试验进一步研究了细胞浸润以及PCL polyHIPE支持血管向内生长的潜力。我们的结果表明，纳米纤维PCL静电纺丝层能够至少四周限制细胞浸润，而PCL polyHIPE支持细胞浸润、骨细胞的钙和矿物质沉积以及通过孔隙的血管向内生长。
