Ren Ying-Kun, Xiao Yan, Wan Xiang-Bin, Zhao Yu-Zhou, Li Jian, Li Yin, Han Guang-Sen, Chen Xiao-Bing, Zou Qi-Yun, Wang Gang-Cheng, Lu Chao-Min, Xu Yong-Chao, Wang You-Cai
Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
Department of Gynecologic Oncology, Affiliated Cancer Hospital of Zhengzhou University China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11458-63. eCollection 2015.
Long non-coding RNA (lncRNA) has an important role in carcinoma progression and prognosis. However, little is known about the pathological role of lncRNA HOTTIP (HOXA transcript at the distal tip) in colorectal cancer (CRC) patients. This study attempted to investigate the association of lncRNA HOTTIP expression with progression and prognosis in CRC patients. LncRNA HOTTIP expression was measured in 156 CRC tissues and 21 adjacent non-malignant tissues using qRT-PCR. In present study, our results indicated that lncRNA HOTTIP was highly expressed in CRC compared with adjacent non-malignant tissues (P<0.001), and positively correlated with T stage (T1-2 vs. T3-4, P = 0.001), clinical stage (I-II stages vs. III-IV stages, P = 0.003), and distant metastasis (absent vs. present, P = 0.014) in CRC patients. Furthermore, we also observed that increased lncRNA HOTTIP expression was an unfavorable prognostic factor in CRC patients (P = 0.001), regardless of T stage, distant metastasis and clinical stage. Finally, overexpression of lncRNA HOTTIP was supposed to be an independent poor prognostic factor for CRC patients through multivariate analysis (P = 0.017). In conclusion, lncRNA HOTTIP overexpression maybe serves as an unfavorable prognosis predictor for CRC patients. However, a further larger sample size investigation is needed to support our results.
长链非编码RNA（lncRNA）在癌症进展和预后中发挥着重要作用。然而，关于lncRNA HOTTIP（HOXA基因座远端转录本）在结直肠癌（CRC）患者中的病理作用，我们所知甚少。本研究旨在探讨lncRNA HOTTIP表达与CRC患者病情进展及预后的关系。采用qRT-PCR检测了156例CRC组织和21例癌旁非恶性组织中lncRNA HOTTIP的表达。在本研究中，我们的结果表明，与癌旁非恶性组织相比，lncRNA HOTTIP在CRC中高表达（P<0.001），并且与CRC患者的T分期（T1-2期与T3-4期，P = 0.001）、临床分期（I-II期与III-IV期，P = 0.003）以及远处转移（无转移与有转移，P = 0.014）呈正相关。此外，我们还观察到lncRNA HOTTIP表达增加是CRC患者预后不良的一个因素（P = 0.001），无论T分期、远处转移和临床分期如何。最后，通过多因素分析，lncRNA HOTTIP的过表达被认为是CRC患者独立的不良预后因素（P = 0.017）。总之，lncRNA HOTTIP的过表达可能是CRC患者预后不良的一个预测指标。然而，需要进一步更大样本量的研究来支持我们的结果。
