Mori Kazusa, Kato Toshihide, Yokota Osamu, Ohtsuka Hiromichi
Rakuno Gakuen University Animal Medical Center, Ebetsu, Hokkaido 069-8501, Japan.
F. SIDE Veterinary Service, Sapporo, Hokkaido 004-0072, Japan.
J Vet Res. 2020 Jun 16;64(2):223-230. doi: 10.2478/jvetres-2020-0042. eCollection 2020 Jun.
The objective of this research was to evaluate the antibody response to multiple doses of an inactivated mixed vaccine against , , and , and to investigate the influence of age at time of vaccination in the field.
Healthy female Holstein calves received the vaccine at the age of 5-12 days and 2, 3, or 4 weeks later in the first experiment or at 1, 2, or 3 weeks of age and 4 weeks later in the second. Blood samples were collected at each vaccination and 3 weeks after the booster dose. Based on the antibody titres after the vaccinations, calves were divided into positive and negative groups for each of the bacteria. Calves in the control group were vaccinated only once at the age of 19-26 days.
Antibody titres against and were significantly increased by the booster. After the second vaccinations, the titres against each bacterium were higher than those of the control group, and the -positive percentage in calves with high maternal antibody levels (MAL) exceeded that in calves with low MAL. In the first experiment, a majority of the -positive calves tended to have received the primary dose at seven days of age or older.
A booster dose of the inactivated bacterial vaccine in young Holstein calves increased antibody production and overcame the maternal antibodies. Calves should be vaccinated first at seven days of age or older.
本研究的目的是评估针对[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]的多剂灭活混合疫苗的抗体反应，并在实际应用中研究接种疫苗时年龄的影响。
在第一个实验中，健康的雌性荷斯坦犊牛在5 - 12日龄时接种疫苗，并在2、3或4周后再次接种；在第二个实验中，犊牛在1、2或3周龄时接种疫苗，并在4周后再次接种。每次接种疫苗时以及加强剂量接种后3周采集血样。根据接种疫苗后的抗体滴度，将犊牛按每种细菌分为阳性和阴性组。对照组的犊牛仅在19 - 26日龄时接种一次疫苗。
加强剂量显著提高了针对[具体细菌名称1]和[具体细菌名称2]的抗体滴度。第二次接种后，针对每种细菌的滴度均高于对照组，且母源抗体水平（MAL）高的犊牛中[具体细菌名称3]阳性百分比超过了MAL低的犊牛。在第一个实验中，大多数[具体细菌名称3]阳性犊牛倾向于在7日龄及以上时接受首剂疫苗接种。
对年轻的荷斯坦犊牛接种一剂灭活细菌疫苗加强针可增加抗体产生并克服母源抗体。犊牛应在7日龄及以上时首次接种疫苗。
