Naessens J, Newson J, McHugh N, Howard C J, Parsons K, Jones B
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Immunology. 1990 Apr;69(4):525-30.
A new bovine B-cell differentiation antigen is described that is detected by three monoclonal antibodies (mAb). The antigen is not an immunoglobulin and is precipitated from peripheral B cells as a molecule with an approximate molecular weight (MW) of 120,000 or 145,000 before and after reduction, respectively. Data obtained from two-colour cytofluorimetry and immunohistochemistry confirmed that the antigen was found only on mature B cells and on cells with dendritic morphology in the follicles of the organized lymphoid tissues. Its level of expression is directly correlated with that of IgM on peripheral blood B cells and Theileria parva-transformed B cells. The marker was also expressed on the peripheral cells which expressed surface IgG. Based on the antigen's cellular distribution, biochemistry and histochemistry, it is considered to be analogous to the human CD21 antigen.
本文描述了一种新的牛B细胞分化抗原，该抗原可被三种单克隆抗体（mAb）检测到。该抗原不是免疫球蛋白，在外周血B细胞中沉淀出来的分子，还原前后的分子量（MW）分别约为120,000和145,000。双色细胞荧光分析和免疫组织化学获得的数据证实，该抗原仅在成熟B细胞以及有组织的淋巴组织滤泡中具有树突状形态的细胞上发现。其表达水平与外周血B细胞和微小泰勒虫转化的B细胞上IgM的表达水平直接相关。该标志物也在外周表达表面IgG的细胞上表达。基于该抗原的细胞分布、生物化学和组织化学特性，认为它类似于人类CD21抗原。
