Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Hospital of Southwest Medical University, Lu Zhou, China.
Department of Health Management Center, The Affiliated Hospital of Southwest Medical University, Lu Zhou, China.
Brain Behav. 2023 Sep;13(9):e3156. doi: 10.1002/brb3.3156. Epub 2023 Aug 7.
INTRODUCTION/AIMS: To facilitate further investigation into the mechanisms of facial nerve regeneration, a simple and reliable model of facial nerve crush injury is essential. Nevertheless, the establishment of such models lacks standardization and repeatability, while the healing capacity of the nerve is often overlooked, potentially affecting future studies.
We made facial nerve trunk crush injury models with different pressing times and detected the changes from the distal nerves to the motoneurons via behavior analysis, electrophysiological test, and histomorphometry analysis.
It revealed a particular capacity for self-healing following facial nerve crush damage because there was almost no facial motoneuron apoptosis in the MC group during the observation period, and rats in MC group had total facial paralysis in behavioral tests following surgery and varying degrees of recovery 28 days postoperatively with no treatments. As the pressing time increased, the latency, wave amplitude, nerve fiber damage degree, nerve axon ratio, myelin thickness, electroneurograph (ENoG) value, ultrastructural damage, abnormal morphological changes, and the buccal muscle atrophy of each MC group gradually increased or got worse during the observation period. However, after 28 postoperative days, only the ENoG values of the M10min and M12min groups were beyond 90%, indicating no self-healing.
It suggests that a stable model of peripheral facial palsy may be created by applying a 12.5 cm mosquito clamped to the facial nerve trunk for at least 10 min, which laid the foundation for the subsequent research to objectively evaluate facial nerve regeneration.
简介/目的：为了促进对面神经再生机制的进一步研究，建立一种简单可靠的面神经压榨损伤模型至关重要。然而，此类模型的建立缺乏标准化和可重复性，同时往往忽视了神经的愈合能力，这可能会影响未来的研究。
我们使用不同压迫时间制作面神经干压榨损伤模型，并通过行为分析、电生理测试和组织形态学分析，从远端神经到运动神经元检测变化。
面神经压榨损伤后存在特定的自我修复能力，因为在观察期内 MC 组几乎没有面神经运动神经元凋亡，且手术后 MC 组大鼠在行为测试中出现完全性面瘫，未经治疗 28 天后逐渐恢复，但存在不同程度的瘫痪。随着压迫时间的增加，潜伏期、波幅、神经纤维损伤程度、神经轴突比、髓鞘厚度、电神经图（ENoG）值、超微结构损伤、形态异常改变以及各 MC 组颊肌萎缩在观察期内逐渐增加或恶化。然而，术后 28 天，仅 M10min 和 M12min 组的 ENoG 值超过 90%，表明无自我修复。
这表明，使用至少 10 分钟的 12.5cm 蚊式钳夹在面神经干上可以建立稳定的周围性面瘫模型，为后续客观评估面神经再生的研究奠定了基础。
