基于伊朗北部马赞德兰省环境土壤样本中GRA6基因的分离与基因型特征分析
Isolation and Genotypic Characterization of Based on GRA6 Gene from Environmental Soil Samples in Mazandaran Province, North of Iran.
作者信息
Haghparast-Kenari Beheshteh, Sarvi Shahabeddin, Sharif Mehdi, Ahmadpour Ehsan, Hosseini Seyed Abdollah, Daryani Ahmad
机构信息
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Toxoplasmosis Research Center (TRC), Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Iran J Parasitol. 2020 Apr-Jun;15(2):158-167.
BACKGROUND
Soil is one of the environmental sources of oocysts. The other hand, genotype of the parasite is one of the important factors for its pathogenicity. Due to the importance of toxoplasmosis on public health, this study aimed to isolation and genotyping of in environmental soil samples of Mazandaran Province, north of Iran.
METHODS
Overall, 192 soil samples were collected from different areas in Mazandaran Province from Apr to Sep 2014. The flotation method was used for recovering oocysts. Then, soil samples were investigated for DNA detection of using nested PCR of RE gene, genotyping with Semi-nested PCR of GRA6 gene and restriction fragment length polymorphism (RFLP) analysis. Results were analyzed using Chi-squared test. A significant difference was considered with a <0.05.
RESULTS
From 192 soil samples, DNA was detected in 150 samples (78.1%). Then genotype of 23 samples was determined (91.3% type I and 8.7% type II).
CONCLUSION
Prevalence of in soil samples of Mazandaran province, north of Iran is high and GRA6 type I is predominant. Soil can be the most important source of severe toxoplasmosis in this province.
背景
土壤是卵囊的环境来源之一。另一方面，寄生虫的基因型是其致病性的重要因素之一。由于弓形虫病对公共卫生的重要性，本研究旨在对伊朗北部马赞德兰省环境土壤样本中的弓形虫进行分离和基因分型。
方法
2014年4月至9月，共从马赞德兰省不同地区采集了192份土壤样本。采用浮选法回收卵囊。然后，使用RE基因的巢式PCR对土壤样本进行弓形虫DNA检测，通过GRA6基因的半巢式PCR和限制性片段长度多态性（RFLP）分析进行基因分型。结果采用卡方检验进行分析。P<0.05被认为有显著差异。
结果
在192份土壤样本中，150份样本（78.1%）检测到弓形虫DNA。然后确定了23份样本的基因型（I型占91.3%，II型占8.7%）。
结论
伊朗北部马赞德兰省土壤样本中弓形虫的感染率很高，且GRA6 I型占主导。在该省，土壤可能是严重弓形虫病的最重要来源。
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