Saki J, Khademvatan S, Yousefi E, Tavalla M, Abdizadeh R
Health Research Institute, Infectious and Tropical Diseases Research Center & Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Parasitology and Mycology and Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, PO Box: 613715794, Iran.
J Parasit Dis. 2017 Mar;41(1):202-205. doi: 10.1007/s12639-016-0778-1. Epub 2016 Apr 12.
To detection and genotype of isolated from soil in Ahvaz, southwest of Iran. Between August 2011 and May 2012 at different sites located in the area of the Ahvaz city south west Iran. A total of 200 soil samples were taken from different points of the region. Oocysts were recovered using the flotation method. Then, PCR reactions targeting the GRA6 gene were performed for specific detection. The positive samples were studied by RFLP (random amplified fragment length polymorphism) using enzymes to confirm the parasite linage. DNA was found in 18 samples. Among them, 12 samples were successfully genotyped as GRA6 type III and 6 as GRA6 Type II. This is the first investigation detecting and genotyping oocyst in environmental soil samples of Ahvaz, South west of Iran. The results of this study indicated that soil contaminated with oocysts especially in public park may play a role in the epidemiology of human toxoplasmosis in southwest of Iran.
对从伊朗西南部阿瓦士土壤中分离出的（某种物质）进行检测和基因分型。在2011年8月至2012年5月期间，于伊朗西南部阿瓦士市地区的不同地点。共从该地区不同点采集了200份土壤样本。使用浮选法回收卵囊。然后，针对GRA6基因进行PCR反应以进行特异性检测。对阳性样本采用酶切随机扩增多态性DNA（RFLP）进行研究以确认寄生虫谱系。在18个样本中发现了DNA。其中，12个样本成功基因分型为GRA6 III型，6个为GRA6 II型。这是首次在伊朗西南部阿瓦士环境土壤样本中检测和基因分型卵囊的调查。本研究结果表明，被卵囊污染的土壤，尤其是在公园，可能在伊朗西南部人类弓形虫病的流行病学中起作用。
