Takagi Daisuke, Lin Waka, Matsumoto Takahiko, Yaginuma Hidekazu, Hemmi Natsuko, Hatada Shigeo, Seo Manabu
Ricoh Company Ltd., Healthcare Business Group, Biomedical Business Center, Kawasaki-city, 210-0821, Japan.
Int J Bioprint. 2019 Jul 1;5(2):208. doi: 10.18063/ijb.v5i2.208. eCollection 2019.
In recent years, bioprinting has emerged as a promising technology for the construction of three-dimensional (3D) tissues to be used in regenerative medicine or screening applications. In the present study, we present the development of an inkjet-based bioprinting system to arrange multiple cells and materials precisely into structurally organized constructs. A novel inkjet printhead has been specially designed for live cell ejection. Droplet formation is powered by piezoelectric membrane vibrations coupled with mixing movements to prevent cell sedimentation at the nozzle. Stable drop-on-demand dispensing and cell viability were validated over an adequately long time to allow the fabrication of 3D tissues. Reliable control of cell number and spatial positioning was demonstrated using two separate suspensions with different cell types printed sequentially. Finally, a process for constructing stratified Mille-Feuille-like 3D structures is proposed by alternately superimposing cell suspensions and hydrogel layers with a controlled vertical resolution. The results show that inkjet technology is effective for both two-dimensional patterning and 3D multilayering and has the potential to facilitate the achievement of live cell bioprinting with an unprecedented level of precision.
近年来，生物打印已成为一种很有前景的技术，可用于构建用于再生医学或筛选应用的三维（3D）组织。在本研究中，我们展示了一种基于喷墨的生物打印系统的开发，该系统可将多种细胞和材料精确排列成结构有序的构建体。一种新型喷墨打印头专为活细胞喷射而设计。液滴形成由压电膜振动与混合运动驱动，以防止细胞在喷嘴处沉淀。在足够长的时间内验证了稳定的按需滴注和细胞活力，以允许制造3D组织。使用依次打印的两种不同细胞类型的单独悬浮液，证明了对细胞数量和空间定位的可靠控制。最后，通过以可控的垂直分辨率交替叠加细胞悬浮液和水凝胶层，提出了一种构建分层千层饼状3D结构的方法。结果表明，喷墨技术对于二维图案化和3D多层化均有效，并且有潜力以前所未有的精度促进活细胞生物打印的实现。
