Department of Biology, Indiana University, Bloomington, Indiana, USA.
Department of Biology, Indiana University, Bloomington, Indiana, USA
J Bacteriol. 2020 Aug 25;202(18). doi: 10.1128/JB.00290-20.
The ancestral strain of NCIB3610 (3610) bears a large, low-copy-number plasmid, called pBS32, that was lost during the domestication of laboratory strain derivatives. Selection against pBS32 may have been because it encodes a potent inhibitor of natural genetic competence (ComI), as laboratory strains were selected for high-frequency transformation. Previous studies have shown that pBS32 and its sibling, pLS32 in subsp. , encode a replication initiation protein (RepN), a plasmid partitioning system (AlfAB), a biofilm inhibitor (RapP), and an alternative sigma factor (SigN) that can induce plasmid-mediated cell death in response to DNA damage. Here, we review the literature on pBS32/pLS32, the genes found on it, and their associated phenotypes.
NCIB3610(3610)的原始菌株带有一个大型低拷贝数质粒,称为 pBS32,该质粒在实验室菌株衍生物的驯化过程中丢失。对 pBS32 的选择可能是因为它编码一种天然遗传能力(ComI)的强抑制剂,因为实验室菌株被选择用于高频转化。先前的研究表明,pBS32 及其在 亚种中的同源物 pLS32 编码复制起始蛋白(RepN)、质粒分配系统(AlfAB)、生物膜抑制剂(RapP)和替代 sigma 因子(SigN),可在响应 DNA 损伤时诱导质粒介导的细胞死亡。在这里,我们回顾了有关 pBS32/pLS32、其上发现的基因及其相关表型的文献。