Ludwig Institute for Cancer Research, Stockholm, Sweden.
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Nat Biotechnol. 2016 Dec;34(12):1264-1266. doi: 10.1038/nbt.3701. Epub 2016 Oct 31.
Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states.
目前对于小 RNA 表达的异质性知之甚少，因为小 RNA 分析迄今为止需要大量的细胞。在这里，我们提出了一种用于小 RNA 测序的单细胞方法，并将其应用于原始和诱导的人类胚胎干细胞和癌细胞。microRNA 以及 tRNA 和小核仁 RNA（snoRNA）片段的分析揭示了 microRNA 作为不同细胞类型和状态标记物的潜力。
