"Petru Poni" Institute of Macromolecular Chemistry, Romanian Academy, Centre of Advanced Research in Bionanoconjugates and Biopolymers, Grigore Ghica Voda Alley 41 A, 700487 Iasi, Romania.
The Research Institute of the University of Bucharest (ICUB), 90 Sos. Panduri, 050663 Bucharest, Romania.
Molecules. 2023 Apr 18;28(8):3561. doi: 10.3390/molecules28083561.
Raman nanoparticle probes are a potent class of optical labels for the interrogation of pathological and physiological processes in cells, bioassays, and tissues. Herein, we review the recent advancements in fluorescent and Raman imaging using oligodeoxyribonucleotide (ODN)-based nanoparticles and nanostructures, which show promise as effective tools for live-cell analysis. These nanodevices can be used to investigate a vast number of biological processes occurring at various levels, starting from those involving organelles, cells, tissues, and whole living organisms. ODN-based fluorescent and Raman probes have contributed to the achievement of significant advancements in the comprehension of the role played by specific analytes in pathological processes and have inaugurated new possibilities for diagnosing health conditions. The technological implications that have emerged from the studies herein described could open new avenues for innovative diagnostics aimed at identifying socially relevant diseases like cancer through the utilization of intracellular markers and/or guide surgical procedures based on fluorescent or Raman imaging. Particularly complex probe structures have been developed within the past five years, creating a versatile toolbox for live-cell analysis, with each tool possessing its own strengths and limitations for specific studies. Analyzing the literature reports in the field, we predict that the development of ODN-based fluorescent and Raman probes will continue in the near future, disclosing novel ideas on their application in therapeutic and diagnostic strategies.
基于拉曼纳米粒子的探针是一类强大的光学标记物，可用于研究细胞、生物测定和组织中的病理和生理过程。本文综述了基于寡脱氧核苷酸 (ODN) 的纳米粒子和纳米结构的荧光和拉曼成像的最新进展，这些进展有望成为活细胞分析的有效工具。这些纳米器件可用于研究在各种水平上发生的大量生物学过程，从涉及细胞器、细胞、组织和整个生物体的过程开始。基于 ODN 的荧光和拉曼探针有助于深入了解特定分析物在病理过程中所起的作用，并为诊断健康状况开辟了新的可能性。本文所述研究中出现的技术意义可能为创新诊断开辟新途径，旨在通过利用细胞内标记物来识别具有社会意义的疾病，如癌症，以及基于荧光或拉曼成像指导手术。在过去五年中，已经开发出了特别复杂的探针结构，为活细胞分析创建了一个多功能工具箱，每种工具都具有其自身的优势和局限性，适用于特定的研究。通过分析该领域的文献报告，我们预测基于 ODN 的荧光和拉曼探针的开发将在不久的将来继续进行，揭示其在治疗和诊断策略中的应用的新想法。
