Directorate for Nuclear Safety and Security, European Commission, Joint Research Centre, Karlsruhe, Germany.
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy.
Adv Exp Med Biol. 2020;1250:49-61. doi: 10.1007/978-981-15-3262-7_4.
A wide variety of hydrogels have been proposed for tissue engineering applications, cell encapsulation, and bioinks for bioprinting applications. Cell-laden hydrogel constructs rely on natural hydrogels such as alginate, agarose, chitosan, collagen, gelatin, fibroin, and hyaluronic acid (HA), as well as on synthetic hydrogels such as poloxamers (Pluronics) and polyethylene glycol (PEG). Alginate has become more and more important in the last years, thanks to the possibility to prepare alginate hydrogels suitable for cell encapsulation mainly because of the mild and reversible cross-linking conditions. In this paper alginate will be described in detail with respect to its chemistry, cross-linking behavior, biocompatibility, manufacturing capacity, and possible modifications.
已经提出了各种各样的水凝胶，用于组织工程应用、细胞包封以及用于生物打印应用的生物墨水。负载细胞的水凝胶构建体依赖于天然水凝胶，如藻酸盐、琼脂糖、壳聚糖、胶原、明胶、丝素和透明质酸 (HA)，以及合成水凝胶，如泊洛沙姆 (Pluronics) 和聚乙二醇 (PEG)。近年来，由于有可能制备适合细胞包封的藻酸盐水凝胶，主要是由于温和且可重复的交联条件，藻酸盐变得越来越重要。本文将详细描述藻酸盐的化学性质、交联行为、生物相容性、制造能力以及可能的修饰。
