Vivian L. Smith Department of Neurosurgery, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
CNS Oncol. 2020 Jun;9(2):CNS57. doi: 10.2217/cns-2020-0007. Epub 2020 Jun 30.
To explore fecal short-chain fatty acids and neurotransmitter alterations in a mouse-glioma model and glioma patients. Liquid chromatography-mass spectrometry and 16S rRNA-sequencing from fecal samples were performed to measure metabolite levels and taxa abundance in mice/humans. Mice underwent GL261 implantation with/without temozolomide. Glioma patients were compared with healthy controls. Glioma altered several short-chain fatty acids and neurotransmitter levels. Reduced 5-hydroxyindoleaceic acid and norepinephrine levels were seen in mice and humans. Interestingly, temozolomide treatment abrogates the effects of glioma on fecal metabolites. Our findings demonstrate the interplay between glioma and the gut-brain axis. Further work is required to identify pathways within the gut-brain axis by which glioma influences and promotes the modulation of fecal metabolites and microbiome.
探讨小鼠脑胶质瘤模型和脑胶质瘤患者粪便中短链脂肪酸和神经递质的变化。通过对粪便样本进行液相色谱-质谱和 16S rRNA 测序，测量小鼠/人类的代谢物水平和分类群丰度。小鼠接受 GL261 植入术，并用/不用替莫唑胺治疗。将脑胶质瘤患者与健康对照进行比较。脑胶质瘤改变了几种短链脂肪酸和神经递质水平。在小鼠和人类中，5-羟色胺酸和去甲肾上腺素水平降低。有趣的是，替莫唑胺治疗可消除脑胶质瘤对粪便代谢物的影响。我们的研究结果表明了脑胶质瘤与肠脑轴之间的相互作用。需要进一步的研究来确定肠脑轴内的途径，通过这些途径脑胶质瘤影响并促进粪便代谢物和微生物组的调节。
