Université de Lyon - Université Claude Bernard Lyon 1, UMR CNRS 5615, Laboratoire des Multimatériaux et Interfaces, 69372 Lyon Cedex 08, France; Assistance Publique-Hôpitaux de Paris, Hôpital Rothschild, Service d'Odontologie, Université de Paris, Faculté dentaire, France.
Université de Lyon - Université Claude Bernard Lyon 1, UMR CNRS 5615, Laboratoire des Multimatériaux et Interfaces, 69372 Lyon Cedex 08, France; Université de Lyon, Université Claude Bernard Lyon 1, Faculté d'Odontologie, 69008 Lyon, France.
Dent Mater. 2020 Sep;36(9):1116-1143. doi: 10.1016/j.dental.2020.03.020. Epub 2020 Jun 27.
In the context of minimally invasive dentistry and tissue conservation, bioactive products are valuable. The aim of this review was to identify, clarify, and classify the methodologies used to quantify the bioactive glasses bioactivity.
Specific search strategies were performed in electronic databases: PubMed, Embase, Cochrane Library, and Scopus. Papers were selected after a review of their title, abstract, and full text. The following data were then examined for final selection: BAG investigated, objectives, criteria, methods, and outcomes.
Sixty-one studies published from 2001 to 2019, were included. The bioactivity of BAG can be evaluated in vitro in contact with solutions, enamel, dentin, or cells. Other studies have conducted in vivo evaluation by BAG contact with dentin and dental pulp. Studies have used various analysis techniques: evaluation of apatite with or without characterization or assessment of mechanical properties. Reprecipitation mechanisms and pulp cell stimulation are treated together through the term 'bioactivity'.
Based on these results, we suggested a classification of methodologies for a better understanding of the bioactive properties of BAG. According to all in vitro studies, BAG appear to be bioactive materials. No consensus has been reached on the results of in vivo studies, and no comparison has been conducted between protocols to assess the bioactivity of other bioactive competitor products.
在微创牙科和组织保存的背景下，生物活性产品具有重要价值。本综述旨在确定、阐明和分类用于量化生物活性玻璃生物活性的方法。
在电子数据库 PubMed、Embase、Cochrane 图书馆和 Scopus 中进行了特定的搜索策略。在审查标题、摘要和全文后，选择论文。然后检查以下数据进行最终选择：研究的 BAG、目标、标准、方法和结果。
纳入了 2001 年至 2019 年发表的 61 项研究。BAG 的生物活性可以在与溶液、牙釉质、牙本质或细胞接触的情况下进行体外评估。其他研究通过 BAG 与牙本质和牙髓的接触进行了体内评估。研究使用了各种分析技术：有无特征评估的磷灰石评估或机械性能评估。再沉淀机制和牙髓细胞刺激通过术语“生物活性”一起处理。
根据这些结果，我们提出了一种分类方法，以更好地理解 BAG 的生物活性特性。根据所有体外研究，BAG 似乎是生物活性材料。对体内研究的结果尚未达成共识，也没有对评估其他生物活性竞争产品生物活性的方案进行比较。
