Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal.
In Vivo. 2020 Jul-Aug;34(4):1797-1803. doi: 10.21873/invivo.11974.
This work intended to improve the knowledge of the rat model of prostate cancer (PCa) by ultrasonographic monitoring.
Male Wistar rats were divided into control (n=8) and PCa (n=14) groups. PCa development was induced in the PCa group through the sequential administration of the anti-androgenic drug flutamide, testosterone propionate and the carcinogenic N-methyl-N-nitrosourea. The prostate was evaluated by ultrasonography at five timepoints along 49 weeks of the experimental protocol. Ventral prostate lobes were observed in all ultrasonographic examinations.
The ventral prostate area of the control group increased gradually between the first and the last ultrasonographic examination. The ventral prostate area of PCa groups decreased due to flutamide administration and increased after androgen and carcinogen administration. The area of the dorsal prostate lobe increased between the fourth and the fifth ultrasonographic examination. In the last ultrasonographic examination, hypoechoic and anechoic lesions were observed in the PCa group.
To our knowledge, this is the first study presenting a follow-up of rat prostatic dimensions by ultrasonography. Ultrasonography is a feasible approach for prostate cancer monitoring in experimental models.
通过超声监测，提高对前列腺癌（PCa）大鼠模型的认识。
雄性 Wistar 大鼠分为对照组（n=8）和 PCa 组（n=14）。在 PCa 组中，通过序贯给予抗雄激素药物氟他胺、丙酸睾酮和致癌物质 N-甲基-N-亚硝脲来诱导 PCa 的发展。在实验方案的 49 周内，通过超声检查五次评估前列腺。所有超声检查均观察腹侧前列腺叶。
对照组的腹侧前列腺面积在第一次和最后一次超声检查之间逐渐增加。由于氟他胺的给予，PCa 组的腹侧前列腺面积减少，在给予雄激素和致癌物后增加。背侧前列腺叶的面积在第四次和第五次超声检查之间增加。在最后一次超声检查中，PCa 组观察到低回声和无回声病变。
据我们所知，这是首次通过超声对大鼠前列腺尺寸进行随访的研究。超声是监测实验模型中前列腺癌的一种可行方法。
