Melbourne Dental School, University of Melbourne, Melbourne, Vic., Australia.
Department of Dermatology, Royal Melbourne Hospital, Melbourne, Vic., Australia.
Oral Dis. 2021 Mar;27(2):378-387. doi: 10.1111/odi.13523. Epub 2020 Jul 27.
To identify factors which influence the intraoral prevalence of human herpes viruses (HHVs) using mucosal swabs, saliva samples and qPCR analysis.
In this cross-sectional observational study, matched saliva and oral swabs were collected from a total of 115 subjects: 70 immunocompetent subjects with no mucosal abnormalities, 22 with mucosal abnormalities and 23 therapeutically immunocompromised individuals. Extracted DNA was analysed by multiplex qPCR for detection and quantification of HHVs 1-6.
At least one human herpes virus was detected in 77.1% of immunocompetent individuals with no mucosal abnormalities, with EBV the most commonly detected at 61.4%. HHV-6 was detected in 17.1%, HSV-1 in 4.3% and CMV in 1.1%. Detection was higher in saliva than in oral swabs. There was no detection of HSV-2 or VZV. Neither presence of oral mucosal abnormality nor therapeutic immunocompromise was related to increased detection of human herpes virus.
Commensal detection rates of EBV are high, and caution in clinical correlation of positive detection is warranted. Commensal CMV rates are low, and detection is likely to be clinically relevant. This study presents a comprehensive commensal detection rate of HHVs 1-6 by qPCR in saliva and swabs.
使用黏膜拭子、唾液样本和 qPCR 分析来确定影响人类疱疹病毒（HHV）口腔内流行率的因素。
在这项横断面观察性研究中，共采集了 115 名受试者的匹配唾液和口腔拭子：70 名免疫功能正常且无黏膜异常的受试者、22 名黏膜异常的受试者和 23 名免疫治疗受损的个体。提取的 DNA 通过多重 qPCR 进行分析，以检测和定量 HHVs 1-6。
在无黏膜异常的免疫功能正常个体中，77.1%至少检测到一种人类疱疹病毒，其中 EBV 最常见，为 61.4%。HHV-6 的检出率为 17.1%，HSV-1 为 4.3%，CMV 为 1.1%。在唾液中的检出率高于口腔拭子。未检测到 HSV-2 或 VZV。口腔黏膜异常的存在或治疗性免疫抑制均与人类疱疹病毒的检出率增加无关。
EBV 的共生检出率较高，因此在临床相关性检测中应谨慎。共生 CMV 的检出率较低，检测结果可能具有临床相关性。本研究通过 qPCR 对唾液和拭子中的 HHVs 1-6 进行了全面的共生检测率分析。
