Mori N, Oka K, Yoda Y, Abe T, Kojima M
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Am J Clin Pathol. 1988 Sep;90(3):244-9. doi: 10.1093/ajcp/90.3.244.
The authors studied Leu-4 (CD3) antigen localization in the neoplastic cells from six cases with T-ALL, eight with T-lymphoblastic lymphoma (T-LL), and eight with peripheral T-cell lymphomas using immunoelectron microscopy (immuno-EM). Other various monoclonal antibody studies were also performed to detect the stages of differentiation of the neoplastic cells light microscopically. The authors' study indicated that cytoplasmic Leu-4 (cLeu-4) antigen expression appeared at the early stage of thymic T-cells, together with Leu-9 (CD7) and T101 (CD5). Their immuno-EM study also disclosed that the neoplastic cells at the early stage of thymocyte differentiation all had positive results for cLeu-4 but negative results for surface Leu-4 (sLeu-4). The Leu-4 antigen expression in the neoplastic cells at the stage of common thymocyte differentiation with OKT6- (CD1) positive phenotype was variable, showing cLeu-4+sLeu-4-, cLeu-4-sLeu-4+, and cLeu-4+sLeu-4+, depending on the cases investigated. From these results, they suggest that cLeu-4 antigen appears at the stage of early thymocyte differentiation, followed by predominant sLeu-4 expression at the stage of common thymocyte differentiation, and finally simultaneous expression of cLeu-4 and sLeu-4 at the stage of peripheral T-cells.
作者使用免疫电子显微镜（免疫电镜）研究了6例T细胞急性淋巴细胞白血病（T-ALL）、8例T淋巴母细胞淋巴瘤（T-LL）和8例外周T细胞淋巴瘤的肿瘤细胞中Leu-4（CD3）抗原的定位。还进行了其他各种单克隆抗体研究，以通过光学显微镜检测肿瘤细胞的分化阶段。作者的研究表明，细胞质Leu-4（cLeu-4）抗原表达出现在胸腺T细胞的早期阶段，与Leu-9（CD7）和T101（CD5）一起。他们的免疫电镜研究还发现，胸腺细胞分化早期的肿瘤细胞cLeu-4均呈阳性，但表面Leu-4（sLeu-4）呈阴性。具有OKT6-（CD1）阳性表型的普通胸腺细胞分化阶段的肿瘤细胞中Leu-4抗原表达各不相同，根据所研究的病例显示为cLeu-4+sLeu-4-、cLeu-4-sLeu-4+和cLeu-4+sLeu-4+。根据这些结果，他们认为cLeu-4抗原出现在胸腺细胞分化的早期阶段，随后在普通胸腺细胞分化阶段主要表达sLeu-4，最后在外周T细胞阶段同时表达cLeu-4和sLeu-4。
