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半乳糖凝集素-8通过促进抗原内化和加工增强T细胞反应。

Galectin-8 Enhances T cell Response by Promotion of Antigen Internalization and Processing.

作者信息

Prato Cecilia Arahí, Carabelli Julieta, Campetella Oscar, Tribulatti María Virginia

机构信息

Laboratorio de Inmunología Molecular, Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires B1650HMP, Argentina.

Laboratorio de Inmunología Molecular, Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires B1650HMP, Argentina.

出版信息

iScience. 2020 Jul 24;23(7):101278. doi: 10.1016/j.isci.2020.101278. Epub 2020 Jun 17.

DOI:10.1016/j.isci.2020.101278
PMID:32619699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7334376/
Abstract

Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on the surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from antigen nature. Both Gal-8 and antigen were together internalized and localized in early endosomes. Interestingly, antigen processing by APCs was also accelerated in the presence of Gal-8 as a separate mechanism, distinct from the increased antigen internalization. Moreover, APCs pulsed together with antigen and Gal-8 were able to activate cognate CD4 T cells more efficiently than those pulsed with antigen alone. This enhanced antigen presentation was still evident in the absence of costimulatory signals and APCs-derived soluble mediators. Therefore, our results provide evidence for as yet unrecognized mechanism by which Gal-8 stimulates the elicitation of the immune response in a lectin-dependent manner, by inducing antigen uptake and processing upon lattice formation at APCs surface.

摘要

半乳糖凝集素-8（Gal-8）是一种具有免疫刺激能力的哺乳动物凝集素。在本研究中，我们证明抗原呈递细胞（APC）表面的Gal-8-聚糖相互作用可促进抗原结合和内化，而与抗原性质无关。Gal-8和抗原一起被内化并定位在早期内体中。有趣的是，作为一种独立于抗原内化增加的机制，在Gal-8存在的情况下，APC的抗原加工也会加速。此外，与抗原和Gal-8一起脉冲处理的APC比仅用抗原脉冲处理的APC更能有效地激活同源CD4 T细胞。在没有共刺激信号和APC衍生的可溶性介质的情况下，这种增强的抗原呈递仍然很明显。因此，我们的结果为一种尚未被认识的机制提供了证据，即Gal-8通过在APC表面形成晶格时诱导抗原摄取和加工，以凝集素依赖的方式刺激免疫反应的引发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/b5b70e501d0c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/5b9c4e88d821/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/25de6075ef9c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/7c93a49ab164/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/6c4db957c835/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/962d09a799f7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/b5b70e501d0c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/5b9c4e88d821/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/25de6075ef9c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/7c93a49ab164/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/6c4db957c835/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/962d09a799f7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a21d/7334376/b5b70e501d0c/gr5.jpg

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