Laboratorio de Inmunología Molecular, Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires B1650HMP, Argentina.
STAR Protoc. 2020 Dec 9;1(3):100204. doi: 10.1016/j.xpro.2020.100204. eCollection 2020 Dec 18.
Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of the recombinant protein is a recurrent strategy for studying their biological functions. Here, we provide a general protocol for the production of Galectins and their isolated or chimeric domains. We take advantage of their lectin activity and the 6xHis-tag addition for purification, thus obtaining a highly pure and active Galectin to use in both and assays. For complete details on the use and execution of this protocol, please refer to Cattaneo et al. (2011), Tribulatti et al. (2012), and Prato et al. (2020).
半乳糖凝集素是可溶性凝集素，参与许多生理和病理功能。由于它们可以在细胞外发挥作用，因此使用重组蛋白是研究其生物学功能的常用策略。在这里，我们提供了一种生产半乳糖凝集素及其分离或嵌合结构域的通用方案。我们利用它们的凝集素活性和 6xHis 标签添加进行纯化，从而获得高度纯和活性的半乳糖凝集素来用于 和 测定。有关此方案的使用和执行的完整详细信息，请参阅 Cattaneo 等人（2011 年）、Tribulatti 等人（2012 年）和 Prato 等人（2020 年）。
