Lamche H R, Paul E, Schlag G, Redl H, Hammerschmidt D E
Ludwig-Boltzmann-Institut für experimentelle Traumatologie, Lorenz Böhler Unfallkrankenhaus, Vienna, Republic of Austria.
Inflammation. 1988 Jun;12(3):265-76. doi: 10.1007/BF00920078.
A radioimmunoassay was devised for the human complement cleavage product, C3a, using charcoal separation and selective precipitation of interfering substances. When compared with the commercially available immunoassay now marketed, the assay reported here was somewhat simpler to perform; furthermore, it overcame delivery and availability problems in Europe. The assay showed a mean recovery of 87% of known amounts of C3a or C3adesarginine and had a sensitivity of 32 ng C3a per milliliter of plasma; coefficients of variance were comparable to other radioimmunoassays in common use. Using this assay in a first clinical application, we were able to document a small but statistically significant rise in [C3a] during cardiopulmonary bypass.
采用活性炭分离和干扰物质选择性沉淀法，设计了一种用于检测人补体裂解产物C3a的放射免疫分析方法。与目前市场上销售的商用免疫分析方法相比，本文报道的分析方法操作起来更为简便；此外，它还克服了欧洲地区产品供应和可获得性方面的问题。该分析方法对已知量的C3a或C3ades精氨酸的平均回收率为87%，血浆中C3a的检测灵敏度为每毫升32纳克；变异系数与其他常用放射免疫分析方法相当。在首次临床应用中使用该分析方法时，我们能够证明在体外循环期间[C3a]有小幅但具有统计学意义的升高。
