Zhang Yan, Tan Luxuan, Li Chao, Wu Han, Ran Dan, Zhang Zhenyu
Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, China.
Department of Gastroenterology, The Second Hospital of Nanjing, Nanjing University of Chineses Medicine, Nanjing, 210003, China.
AMB Express. 2020 Jul 3;10(1):119. doi: 10.1186/s13568-020-01053-z.
Sulforaphane (SFN) is a kind of natural isothiocyanate, which exists in cruciferous plants. Only few studies were about the anti-inflammatory effects of sulforaphane in ulcerative colitis. In this study, our purpose is to explore the effects of sulforaphane on the intestinal microbial community of UC mice. The severity of mice colitis were measured by colon length, survial rate, body weight and disease activity index (DAI) score. Histological and morphological evaluation of colon tissues were performed by HE. 16S rRNA gene amplicon pyrosequencing was used to analyza the changes of mouse flora. The variety of flora expression were explored using quantitative PCR. Sulforaphane treated mice had larger body weight and longer colon length than DSS-induced mice. The colon tissues of DSS group showed congestion and edema. Meanwhile, treatment with sulforaphane effectively reducted the damage scores and MPO activity. Sulforaphane reversed DSS-induced gut dysbiosis. Sulforaphane would shift the balance to Butyricicoccus on inflammation. The possible anti-inflammatory mechanism of sulforaphane is to coordinate with the probiotics such as Butyricicoccus. In summary, these findings proved that sulforaphane might be a useful content and serve as a potential therapy in the treatment of UC.
萝卜硫素（SFN）是一种天然异硫氰酸盐，存在于十字花科植物中。关于萝卜硫素在溃疡性结肠炎中的抗炎作用，仅有少数研究。在本研究中，我们的目的是探究萝卜硫素对溃疡性结肠炎（UC）小鼠肠道微生物群落的影响。通过结肠长度、存活率、体重和疾病活动指数（DAI）评分来衡量小鼠结肠炎的严重程度。采用苏木精-伊红（HE）染色对结肠组织进行组织学和形态学评估。利用16S rRNA基因扩增子焦磷酸测序分析小鼠菌群的变化。运用定量PCR探究菌群表达的变化。与硫酸葡聚糖钠（DSS）诱导的小鼠相比，经萝卜硫素处理的小鼠体重更大，结肠长度更长。DSS组的结肠组织出现充血和水肿。同时，萝卜硫素治疗有效降低了损伤评分和髓过氧化物酶（MPO）活性。萝卜硫素逆转了DSS诱导的肠道菌群失调。在炎症状态下，萝卜硫素会使菌群平衡向丁酸球菌属转变。萝卜硫素可能的抗炎机制是与丁酸球菌属等益生菌协同作用。总之，这些研究结果证明萝卜硫素可能是一种有益成分，并有望成为治疗溃疡性结肠炎的潜在疗法。
