姜黄素和水飞蓟宾对暴露于赭曲霉毒素A、伏马菌素B和脱氧雪腐镰刀菌烯醇的PK-15细胞的细胞保护作用。

Cytoprotective effects of curcumin and silymarin on PK-15 cells exposed to ochratoxin A, fumonisin B and deoxynivalenol.

作者信息

Ledur Pauline Christ, Santurio Janio M

机构信息

Programa de Pós-graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, Brazil.

出版信息

Toxicon. 2020 Oct 15;185:97-103. doi: 10.1016/j.toxicon.2020.06.025. Epub 2020 Jul 2.

DOI:10.1016/j.toxicon.2020.06.025

PMID:32622693

Abstract

Mycotoxins are toxic secondary metabolites produced by fungus which cause worldwide concern regarding food and feed safety. Ochratoxin A (OTA), fumonisin B (FB) and deoxynivalenol (DON) are some of the main mycotoxins and oxidative stress is the main mechanism of toxicity. Thereby, this study investigates the in vitro cytoprotective effects of curcumin (CUR) and silymarin (SIL) - known for their strong antioxidant activity - in PK-15 cells exposed to OTA, FB and DON. Pretreatment with CUR and SIL enhanced the viability of cells exposed to the mycotoxins (P < 0.001) and attenuated reactive oxygen species (ROS) formation by DON (P < 0.01), partially reduced ROS formation by FB (P < 0.001), but not OTA. CUR significantly decreased apoptosis in cells exposed to DON (P < 0.01) but was not able to prevent apoptosis in cells exposed to OTA and FB. Whereas SIL was able to prevent apoptosis in PK-15 cells exposed to FB and DON (P < 0.01) but was not able to decrease apoptosis in cells exposed to OTA. In summary, these data indicate that curcumin and silymarin are able to provide cytoprotection against toxicity induced by OTA, FB and DON in PK-15 cells.

摘要

霉菌毒素是由真菌产生的有毒次生代谢产物，引发了全球对食品和饲料安全的关注。赭曲霉毒素A（OTA）、伏马菌素B（FB）和脱氧雪腐镰刀菌烯醇（DON）是一些主要的霉菌毒素，氧化应激是主要的毒性机制。因此，本研究调查了姜黄素（CUR）和水飞蓟素（SIL）——因其强大的抗氧化活性而闻名——对暴露于OTA、FB和DON的PK-15细胞的体外细胞保护作用。用CUR和SIL预处理可提高暴露于霉菌毒素的细胞的活力（P < 0.001），并减弱DON诱导的活性氧（ROS）形成（P < 0.01），部分降低FB诱导的ROS形成（P < 0.001），但对OTA无效。CUR显著降低暴露于DON的细胞中的凋亡（P < 0.01），但不能预防暴露于OTA和FB的细胞中的凋亡。而SIL能够预防暴露于FB和DON的PK-15细胞中的凋亡（P < 0.01），但不能降低暴露于OTA的细胞中的凋亡。总之，这些数据表明姜黄素和水飞蓟素能够为PK-15细胞提供针对OTA、FB和DON诱导的毒性的细胞保护作用。