Dirscherl Cindy, Springer Sebastian
Department of Life Sciences and Chemistry Jacobs University Bremen Germany.
Eng Life Sci. 2017 Sep 15;18(2):124-131. doi: 10.1002/elsc.201700010. eCollection 2018 Feb.
Micrometer-sized patterns of proteins on glass or silica surfaces are in widespread use as protein arrays for probing with ligands or recombinant proteins. More recently, they have been used to capture the surface proteins of mammalian cells seeded onto them, and to arrange these surface proteins into pattern structures. Binding of small molecule ligands or of other proteins, transmembrane or intracellular, to these captured surface proteins can then be quantified. However, reproducible production of protein micropatterns on surfaces can be technically difficult. In this review, we outline the wide potential and the current practical uses of printed protein micropatterns in a historical overview, and we detail some potential pitfalls and difficulties from our own experience, as well as ways to circumvent them.
玻璃或二氧化硅表面上微米级的蛋白质图案作为蛋白质阵列被广泛用于与配体或重组蛋白进行相互作用研究。最近，它们被用于捕获接种在其上的哺乳动物细胞的表面蛋白，并将这些表面蛋白排列成图案结构。然后可以对小分子配体或其他蛋白质（跨膜或细胞内）与这些捕获的表面蛋白的结合进行定量。然而，在表面上可重复地生产蛋白质微图案在技术上可能具有挑战性。在这篇综述中，我们在历史概述中勾勒了印刷蛋白质微图案的广泛潜力和当前实际用途，并根据我们自己的经验详细介绍了一些潜在的陷阱和困难，以及规避这些问题的方法。
