Guo Yan-Hong, Wang Fei-Yan, You Hua-Qian, Wei Yu-Kun, Yang Zong-Qi, Liang Zong-Suo, Yang Dong-Feng
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Science and Medicine,Zhejiang Sci-tech University Hangzhou 310000, China.
Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden Shanghai 201602, China.
Zhongguo Zhong Yao Za Zhi. 2020 Jun;45(11):2509-2514. doi: 10.19540/j.cnki.cjcmm.20200329.112.
Salvia miltiorrhiza(Sm) and Salvia castanea f. tomentosa(Sc) hairy roots were used as experimental materials to study the effects of six different carbon sources, galactose, fructose, lactose, glucose, arabinose and sucrose(control), on fresh weight, dry weight, contents and yields of salvianolic acids and tanshinones. The results showed that galactose was most beneficial to the growth of two kinds of hairy roots, while lactose and arabinose were not conducive to their growth. As for Sm hairy roots, fructose significantly promoted the accumulation of salvianolic acid B, and the content increased by 5.801 times and 10.151 times compared with the control group, respectively. Glucose significantly promoted the accumulation of salvianolic acids. The content and yield of rosmarinic acid were 7.674 times and 9.260 times of that of the control group, and the content and yield of salvianolic acid B were 5.532 times and 6.675 times of the control group. For the hairy roots of Sc, galactose significantly increased the content and yield of rosmarinic acid, reaching 7.820 times and 9.944 times of the control group, respectively. Fructose promoted the increase of the content and yield of cryptotanshinone, reaching 9.242 times and 6.609 times of the control group, respectively. The study confirmed the optimal carbon source for the hairy root culture of Sm and Sc, and provided theoretical guidance for large-scale production of Sm drug-derived components and the utilization of Sc.
以丹参（Sm）和绒毛鼠尾草（Sc）的毛状根为实验材料，研究了半乳糖、果糖、乳糖、葡萄糖、阿拉伯糖和蔗糖（对照）六种不同碳源对丹参酚酸和丹参酮的鲜重、干重、含量及产量的影响。结果表明，半乳糖最有利于两种毛状根的生长，而乳糖和阿拉伯糖不利于其生长。对于Sm毛状根，果糖显著促进了丹酚酸B的积累，其含量分别比对照组增加了5.801倍和10.151倍。葡萄糖显著促进了丹参酚酸的积累。迷迭香酸的含量和产量分别是对照组的7.674倍和9.260倍，丹酚酸B的含量和产量分别是对照组的5.532倍和6.675倍。对于Sc毛状根，半乳糖显著提高了迷迭香酸的含量和产量，分别达到对照组的7.820倍和9.944倍。果糖促进了隐丹参酮含量和产量的增加，分别达到对照组的9.242倍和6.609倍。该研究确定了Sm和Sc毛状根培养的最佳碳源，为Sm药物衍生成分的大规模生产和Sc的利用提供了理论指导。
